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POLR2E Recombinant Protein

CAT: 0793-32-2695-20Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0793-32-2695-20Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Source : E.coli. POLR2E Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 233 amino acids (1-210) and having a molecular mass of 27.1kDa.POLR2E is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. DNA-directed RNA polymerases I, II, and III subunit RPABC1 (POLR2E) is a member of the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family. POLR2E being a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the 4 ribonucleoside triphosphates as substrates. POLR2E is the 5th largest subunit of RNA polymerase II (the polymerase which is responsible for synthesizing messenger RNA in eukaryotes) . The POLR2E subunit is shared by the other 2 DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between the POLR2E subunit and a hepatitis virus transactivating protein has been established, suggesting that an interaction between transcriptional activators and the polymerase can occur through the POLR2E subunit.
Product Name Alternative
DNA-directed RNA polymerases I||II||and III subunit RPABC1||RNA polymerases I||II||and III subunit ABC1||DNA-directed RNA polymerase II 23 kDa polypeptide||DNA-directed RNA polymerase II subunit E||RPB5 homolog||XAP4||POLR2E||RPB5||RPABC1||hRPB25
Purification
Greater than 90% as determined by SDS-PAGE.
Components
The POLR2E solution (1mg/mL) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 1mM DTT.
Storage Conditions
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.
Amino Acids
MGSSHHHHHH SSGLVPRGSH MGSMDDEEET YRLWKIRKTI MQLCHDRGYL VTQDELDQTL EEFKAQFGDK PSEGRPRRTD LTVLVAHNDD PTDQMFVFFP EEPKVGIKTI KVYCQRMQEE NITRALIVVQ QGMTPSAKQS LVDMAPKYIL EQFLQQELLI NITEHELVPE HVVMTKEEVTELLARYKLRE NQLPRIQAGD PVARYFGIKR GQVVKIIRPS ETAGRYITYR LVQ.

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