POLR2E (NM_002695) Human Recombinant Protein

• Background:

  This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

• Synonyms:

  HRPB25; hsRPB5; RPABC1; RPB5; XAP4

• Gene ID:

  5434

• UniProt:

  P19388

• Accession Number mRNA:

  NM_002695

• Chromosomal Location:

  19p13.3

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase

• Field of Research:

  Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Transcription Factors

• Molecular Weight:

  24.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  POLR2E

• Species:

  Human

• Protein ID:

  NP_002686

• Overview:

  Recombinant protein of human polymerase (RNA) II (DNA directed) polypeptide E, 25kDa (POLR2E)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/5434

• Uniprot URL:

  https://www.uniprot.org/uniprot/P19388

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_002695

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_002686