Phospho-GCN2 (T899) Recombinant Rabbit Monoclonal Antibody [JE00-51]

Phospho-GCN2 (T899) Recombinant Rabbit Monoclonal Antibody [JE00-51]

• Description: The family of stress-responsive protein kinases include HRI (heme-regulated inhibitor or EIF2AK1), PKR (EIF2AK2 or TIK), PERK (EIF2AK3) and GCN2 (EIF2AK4). These proteins phosphorylate the eukaryotic translation initiation factor 2α (eIF2α) on Ser 51 to regulate general and gene-specific protein synthesis. Phosphorylated eIF2α acts as an inhibitor of its guanine nucleotide exchange factor eIF2B. GCN2, a unique eIF2α kinase, exists in all eukaryotes from yeast to mammals. In mammals, expression of GCN2 is highest in liver and brain tissues. GCN2 primarily initiates the phosphorylation of eIF2α in response to UV, but has been shown to increase phosphorylation activity in response to serum starvation. Also, substitution of Asp 83 for Ala on eIF2α results in impaired phosphorylation by GCN2 and PKR, suggesting a contribution of remote residues to kinase-substrate recognition.
• Product Name Alternative: E2AK4_HUMAN antibody Eif2ak4 antibody Eukaryotic Translation Initiation Factor 2 alpha kinase 4 antibody Eukaryotic translation initiation factor 2-alpha kinase 4 antibody GCN2 antibody GCN2 eIF2alpha kinase antibody GCN2 like protein antibody GCN2-like protein antibody KIAA1338 antibody MGCN2 antibody
• Abbreviation: EIF2AK4, GCN2, KIAA1338
• CAS Number: 9000-83-3
• Swiss Prot: Q9P2K8 Human,
• Cellular Locus: Cytoplasm.
• Host: Rabbit
• Species Reactivity: Human, Rat
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr899 of Human GCN2.
• Isotype: IgG
• Clone: JE00-51
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB
• Positive Control: HeLa treated with 100nM Calyculin A for 30 minutes cell lysate, C6 treated with 100nM Calyculin A for 30 minutes cell lysate.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 187 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes