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IL17A & IL17F Protein (Native, Human)

CAT: 1543-P525695Size: 100 µgDry Ice: NoHazardous: No
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CAT#:1543-P525695Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Abbreviation
IL17A & IL17F
Host
E. coli
Tag
Native
Purity
> 95 % as determined by SDS-PAGE.
Bioactivity
1.Measured by its ability to induce IL-6 secretion by NIH‐3T3 mouse embryonic fibroblast cells in the presense of 20ng/mL TNFα. The ED50 for this effect is 1-6 ng/mL. 2.Ixekizumab captured on Protein A chip can bind Recombinant Human IL17A & IL17F Heterodimer Protein (Cat. No. CT047-HNAE) with an affinity constant of 5.94 pM as determined in an SPR assay (Biacore T200) (Routinely tested) . 3.Loaded Recombinant Human IL-17RA Protein hFc Tag (Cat. No. 10895-H02H) on ProA Biosensor, can bind Recombinant Human IL17A & IL17F Heterodimer Protein (Cat. No. CT047-HNAE) with an affinity constant of 6.124 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .
Function
Ile20-Ala155 & Arg31-Gln163
Molecular Weight
The recombinant heterodimer of human IL17A & IL17F comprises 271 (137+134) amino acids and has a calculated molecular mass of 30.7 (15.7+15) kDa.
Species
Human

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