TNF Protein (Native, Human)

CAT: 1543-P520340Size: 100 µgDry Ice: NoHazardous: No

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CAT#:1543-P520340Size:100 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Abbreviation

TNF

Synonyms

DIF, TNF-alpha, TNFA, TNFSF2, TNLG1F

Host

E. coli

Tag

Native

Endotoxin

< 5 EU per mg of the protein.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL. The specific activity of recombinant human TNF-alpha is >36,000IU/μg, which is calibrated against the human TNF-alpha WHO International Standard (NIBSC code: 12/154) .

Function

Val77-Leu233

Molecular Weight

The recombinant human TNF-α consists of 158 amino acids and has a predicted molecular mass of 17.5 kDa.

Species

Human

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TNF Protein (Native, Human)

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