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EvaGreen®, 2000X in DMSO

CAT: 0037-#31019Size: 1x 50 µLDry Ice: NoHazardous: No
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CAT#:0037-#31019Size:1x 50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
EvaGreen® Dye, 2000X in DMSO is offered for customers with specialized applications requiring a concentrated dye solution.    Note: for most qPCR applications, we recommend our ready-to-use Forget-Me-Not™ EvaGreen® qPCR Master Mixes or EvaGreen® Dye, 20X in water.    EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR® Green I, making it readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen® dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. EvaGreen® dye is nonmutagenic and noncytotoxic because it is impermeable to cell membranes, unlike SYBR® Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer.    The unique properties of EvaGreen® dye have made it particularly useful in quantitative real-time PCR (qPCR) applications. Compared with the widely used SYBR® Green I, EvaGreen® dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen® dye can be used at a much higher dye concentration than SYBR® Green I, resulting in a high resolution signal for droplet digital PCR, real-time PCR and melt curve analysis.    EvaGreen® dye and applications are covered under granted and pending US and international patents.

Key Features
  • 2000X Concentrated Formulation → Highly concentrated EvaGreen® dye in DMSO for specialized applications requiring flexible dye preparation.
  • High Fluorescence Upon DNA Binding → Essentially non-fluorescent in solution but becomes highly fluorescent when bound to double-stranded DNA.
  • Low PCR Inhibition → EvaGreen® is generally less inhibitory to PCR than SYBR® Green I, allowing higher dye concentrations in suitable amplification workflows.
  • High-Sensitivity DNA Detection → Supports strong fluorescence signals for real-time PCR, melt curve analysis and digital PCR applications.
  • FAM / SYBR® Green I-Compatible Spectrum → DNA-bound EvaGreen® has excitation and emission spectra close to fluorescein (FAM) and SYBR® Green I, supporting compatibility with instruments using appropriate detection channels.
  • Excellent Stability → EvaGreen® exhibits high thermal and hydrolytic stability, supporting routine laboratory handling.
  • Suitable for Specialized Applications → Designed for workflows including qPCR, DNA melt curve analysis, tHDA and capillary gel electrophoresis.
  • Nonmutagenic and Noncytotoxic → EvaGreen® has been reported as nonmutagenic and noncytotoxic based on manufacturer-reported testing.
UNSPSC
41106307
Origin
Synthetic
Applications
EvaGreen® Dye 2000X Applications
  • Real-Time PCR (qPCR) → Fluorescent monitoring and quantification of DNA amplification.
  • DNA Melt Curve Analysis → Analysis of DNA melting behavior following amplification.
  • Droplet Digital PCR (ddPCR) → High-resolution fluorescence-based detection of DNA in partitioned PCR reactions.
  • Thermophilic Helicase-Dependent Amplification (tHDA) → Real-time fluorescence monitoring of DNA amplification using helicase-dependent amplification.
  • Capillary Gel Electrophoresis → Fluorescent nucleic acid detection in compatible capillary electrophoresis workflows.
Stability
12 months
Storage Conditions
R
FAQ
What is EvaGreen® Dye 2000X in DMSO? → EvaGreen® Dye 2000X in DMSO is a highly concentrated fluorescent DNA-binding dye formulated in DMSO for specialized nucleic acid detection and amplification applications. What is EvaGreen® 2000X used for? → It can be used for qPCR, DNA melt curve analysis, droplet digital PCR, thermophilic helicase-dependent amplification and capillary gel electrophoresis. Why is EvaGreen® supplied at 2000X? → The highly concentrated formulation provides flexibility for applications requiring a concentrated EvaGreen® stock solution. It is intended primarily for specialized applications rather than convenience-oriented routine qPCR. Is EvaGreen® an alternative to SYBR® Green I? → Yes. EvaGreen® can serve as an alternative DNA-binding fluorescent dye for qPCR and related applications. It has a similar spectral profile to SYBR® Green I while offering lower PCR inhibition in appropriate workflows. What is the difference between EvaGreen® 2000X in DMSO and EvaGreen® 20X in water? → The main difference is the formulation and concentration. EvaGreen® 2000X is supplied as a highly concentrated DMSO stock for specialized applications, while EvaGreen® 20X in Water provides a lower-concentration aqueous formulation intended for convenient use in common qPCR workflows. What is the size of this EvaGreen® product? → This product is supplied as 1 × 50 µL under Gentaur CAT: 0037-31019.
Classification
Dimethylsulfoxide >99%, CAS 67-68-5
Product MSDS
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