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Human recombinant LIGHT protein, AF

CAT: 0519-PROTO43557-3-100ugSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0519-PROTO43557-3-100ugSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
LIGHT, also known as TNFSF14 is a member of the TNF superfamily that produced by multiple immune cells such as activated T cells and immature dendritic cells (DCs) . LIGHT is a 29 kDa type II transmembrane protein, which serves as a ligand for both lymphotoxin β receptor (LTβR) and TNFSF signaling receptors (TNFRSF14/HVEM) . Besides, LIGHT can amplify NF-kB signaling pathway in T cells when presenting anti-CD3 antibody treatment. Additionally, LIGHT can stimulate T cell proliferation and IFN-expression via interacting with TNFRS13 /HVEM.
Synonyms
TNFSF14, HVEM-L, CD258, LTg
Gene Name
TNFSF14
UniProt
O43557
Reactivity
Human
Tag
His Tag (C-term)
Source
Escherichia coli
Applications
Cell Culture
Purification
>98% as determined by SDS-PAGE.
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Bioactivity
Measure by its ability to induce cytotoxicity in HT-29 cells in the presence of IFN-gamma. The ED50 for this effect is < 10 ng/mL.
Form
The protein was lyophilized from a 0.2 μm filtered solution containing 0.1% sarkosyl in 1X PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Molecular Weight
The protein has a calculated MW of 20.30 kDa. The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis) .
Storage Conditions
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
AA Sequence
MRLGEMVTRLPDGPAGSWEQLIQERRSHEVNPAAHLTGANSSLTGSGGPLLWETQLGLAFLRGLSYHDGALVVTKAGYYYIYSKVQLGGVGCPLGLASTITHGLYKRTPRYPEELELLVSQQSPCGRATSSSRVWWDSSFLGGVVHLEAGEKVVVRVLDERLVRLRDGTRSYFGAFMV with polyhistidine tag at the C-terminus.

UniProtKB · O43557

Tumor necrosis factor ligand superfamily member 14

TNF14_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O43557
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
TNFSF14
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A8K7M2, C9J5H4, O75476, Q6FHA1, Q8WVF8, Q96LD2
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneCytoplasmMembraneSecreted

Molecular function

Cytokine

PTM

Disulfide bondGlycoprotein

Domain

Signal-anchorTransmembraneTransmembrane helix

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