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Mouse recombinant LIGHT protein, AF

CAT: 0519-PROTQ9QYH9-2-5ugSize: 5 µgDry Ice: NoHazardous: No
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CAT#:0519-PROTQ9QYH9-2-5ugSize:5 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
LIGHT, also known as TNFSF14 is a member of the TNF superfamily that produced by multiple immune cells such as activated T cells and immature dendritic cells (DCs) . Mouse LIGHT shares 79% sequence homology with human LIGHT. LIGHT is a 29 kDa type II transmembrane protein, which serves as a ligand for both lymphotoxin β receptor (LTβR) and TNFSF signaling receptors (TNFRSF14/HVEM) . Besides, LIGHT can amplify NF-kB signaling pathway in T cells when presencing anti-CD3 antibody treatment. Additionally, LIGHT is able to stimulate T cell proliferation and IFN expression via interacting with TNFRS13 /HVEM.
Synonyms
TNFSF14, HVEM-L, CD258, Ly113, LTg
Gene Name
Tnfsf14
UniProt
Q9QYH9
Reactivity
Mouse
Tag
His Tag (C-term)
Source
Escherichia coli
Applications
Cell Culture
Purification
>98% as determined by SDS-PAGE.
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Bioactivity
Measure by its ability to induce cytotoxicity in HT-29 cells. The ED50 for this effect is <2 μg/mL.
Form
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Molecular Weight
The protein has a calculated MW of 20.92 kDa. The protein migrates about 17 kDa under reducing condition (SDS-PAGE analysis) .
Storage Conditions
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
AA Sequence
MRLHQRLGDIVAHLPDGGKGSWEKLIQDQRSHQANPAAHLTGANASLIGIGGPLLWETRLGLAFLRGLTYHDGALVTMEPGYYYVYSKVQLSGVGCPQGLANGLPITHGLYKRTSRYPKELELLVSRRSPCGRANSSRVWWDSSFLGGVVHLEAGEEVVVRVPGNRLVRPRDGTRSYFGAFMV with polyhistidine tag at the C-terminus.

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