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Mouse PLTP ELISA Kit PicoKine®

CAT: 0519-EK1368Size: 96 Well With Removable StripsDry Ice: NoHazardous: No
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CAT#:0519-EK1368Size:96 Well With Removable Strips
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Background
Phospholipid transfer protein (PLTP), also known as lipid transfer protein II is a protein that in humans is encoded by the PLTP gene. This gene is mapped to 20q13.12. The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL) . In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene.
Description
Mouse PLTP ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Pltp in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Reproducibility
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1418 1318 1305 1394 1358 48.22 3.5% Sample 2 4309 4364 4776 4611 4515 188.79 4.1% Sample 3 22189 22076 22370 22574 22302 188.71 0.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1418 1318 1305 1394 1358 48.22 3.5% Sample 1 1418 1318 1305 1394 1358 48.22 3.5% Sample 2 4309 4364 4776 4611 4515 188.79 4.1% Sample 2 4309 4364 4776 4611 4515 188.79 4.1% Sample 3 22189 22076 22370 22574 22302 188.71 0.8% Sample 3 22189 22076 22370 22574 22302 188.71 0.8%
Synonyms
Phospholipid transfer protein; Lipid transfer protein II; Pltp
Gene Name
PLTP
Gene ID
18830
UniProt
P55065
Reactivity
Mouse
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: NS0; Immunogen sequence: E18-A493
Capture Antibody
Monoclonal antibody from rat
Detection Antibody
Polyclonal antibody from goat
Tissue Specificity
Highest in lung, adipose tissue, brain, and heart.
Applications
ELISA
Field of Research
Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Cholesterol Metabolism, Heart Disease, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Pathways and Processes, Serum Proteins, Signal Transduction
Standard
50 ng/Tube
Sample Type
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
Detection Range
0.78 ng/mL - 50 ng/mL
Precision
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1418 4309 22189 1296 4212 21284 Standard deviation 87.91 284.39 1020.69 99.79 320.11 1213.18 CV (%) 6.2% 6.6% 4.6% 7.7% 7.6% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1418 4309 22189 1296 4212 21284 Standard deviation 87.91 284.39 1020.69 99.79 320.11 1213.18 CV (%) 6.2% 6.6% 4.6% 7.7% 7.6% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1418 4309 22189 1296 4212 21284 Mean (pg/ml) 1418 4309 22189 1296 4212 21284 Standard deviation 87.91 284.39 1020.69 99.79 320.11 1213.18 Standard deviation 87.91 284.39 1020.69 99.79 320.11 1213.18 CV (%) 6.2% 6.6% 4.6% 7.7% 7.6% 5.7% CV (%) 6.2% 6.6% 4.6% 7.7% 7.6% 5.7%
Sensitivity
<20 pg/mL
Function
Facilitates the transfer of a spectrum of different lipid molecules, including diacylglycerol, phosphatidic acid, sphingomyelin, phosphatidylcholine, phosphatidylglycerol, cerebroside and phosphatidyl ethanolamine. Essential for the transfer of excess surface lipids from triglyceride-rich lipoproteins to HDL, thereby facilitating the formation of smaller lipoprotein remnants, contributing to the formation of LDL, and assisting in the maturation of HDL particles. PLTP also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion. Two distinct forms of PLTP exist in plasma: an active form that can transfer PC from phospholipid vesicles to high-density lipoproteins (HDL), and an inactive form that lacks this capability (By similarity) . .
Components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular Weight
54453 MW
Specificity
Natural and recombinant mouse PLTP
Anticoagulant
Heparin or EDTA
Gene Name Synonym
Phospholipid transfer protein
Subcellular Location
Secreted.
Protein Name
Phospholipid transfer protein
Sample Data
Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.006 0.046 0.093 0.187 0.345 0.743 1.247 2.004 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.006 0.046 0.093 0.187 0.345 0.743 1.247 2.004 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.006 0.046 0.093 0.187 0.345 0.743 1.247 2.004 O.D. 0.006 0.046 0.093 0.187 0.345 0.743 1.247 2.004
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.

UniProtKB · P55065

Phospholipid transfer protein

PLTP_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P55065
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Pltp
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q99L70
Protein keywords

PTM

Disulfide bondGlycoprotein

Biological process

Lipid transportTransport

Cellular component

NucleusSecreted

Technical term

Reference proteome

Domain

Signal

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