Products for Research Use Only
RegisterLogin

Mouse FLRT2 ELISA Kit PicoKine®

CAT: 0519-EK1972Size: 96 Well With Removable StripsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0519-EK1972Size:96 Well With Removable Strips
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Background
Fibronectin leucine-rich repeat transmembrane protein FLRT2 is a protein that in humans is encoded by the FLRT2 gene. It is mapped to 12; 12 E. This gene encodes a member of the fibronectin leucine rich transmembrane (FLRT) family of cell adhesion molecules, which regulate early embryonic vascular and neural development. The encoded type I transmembrane protein has an extracellular region consisting of an N-terminal leucine-rich repeat domain and a type 3 fibronectin domain, followed by a transmembrane domain and a short C-terminal cytoplasmic tail domain. It functions as both a homophilic cell adhesion molecule and a heterophilic chemorepellent through its interaction with members of the uncoordinated-5 receptor family. Proteolytic removal of the extracellular region controls the migration of neurons in the developing cortex. Alternative splicing results in multiple transcript variants.
Description
Mouse FLRT2 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Flrt2 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Reproducibility
Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 52 54 54 56 54 1.65 3.0% Sample 2 239 236 264 250 247 10.98 4.4% Sample 3 887 892 907 888 893 8.01 0.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 52 54 54 56 54 1.65 3.0% Sample 1 52 54 54 56 54 1.65 3.0% Sample 2 239 236 264 250 247 10.98 4.4% Sample 2 239 236 264 250 247 10.98 4.4% Sample 3 887 892 907 888 893 8.01 0.8% Sample 3 887 892 907 888 893 8.01 0.8%
Synonyms
Leucine-rich repeat transmembrane protein FLRT2; Fibronectin-like domain-containing leucine-rich transmembrane protein 2; Flrt2; Kiaa0405
Gene Name
Flrt2
Gene ID
399558
UniProt
Q8BLU0
Reactivity
Mouse
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: NS0; Immunogen sequence: C36-T660
Capture Antibody
A monoclonal antibody from rat
Detection Antibody
A biotinylated detection polyclonal antibody from goat
Tissue Specificity
Detected in adult brain.
Applications
ELISA
Field of Research
Growth Factors/Hormones, Mesenchymal Stem Cells, Signal Transduction, Stem Cells
Standard
10 ng/Tube
Sample Type
Cell culture supernatants, serum and plasma (heparin, EDTA) .
Detection Range
31.2 pg/mL - 2,000 pg/mL
Precision
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 52 239 887 56 265 904 Standard deviation 2.18 9.80 39.92 3.02 11.40 50.62 CV (%) 4.2% 4.1% 4.5% 5.4% 4.3% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 52 239 887 56 265 904 Standard deviation 2.18 9.80 39.92 3.02 11.40 50.62 CV (%) 4.2% 4.1% 4.5% 5.4% 4.3% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 52 239 887 56 265 904 Mean (pg/ml) 52 239 887 56 265 904 Standard deviation 2.18 9.80 39.92 3.02 11.40 50.62 Standard deviation 2.18 9.80 39.92 3.02 11.40 50.62 CV (%) 4.2% 4.1% 4.5% 5.4% 4.3% 5.6% CV (%) 4.2% 4.1% 4.5% 5.4% 4.3% 5.6%
Sensitivity
<10 pg/mL
Function
Functions in cell-cell adhesion, cell migration and axon guidance. Mediates cell-cell adhesion via its interactions with ADGRL3 and probably also other latrophilins that are expressed at the surface of adjacent cells. May play a role in the migration of c
Components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Specificity
Natural and recombinant mouse FLRT2
Anticoagulant
Heparin or EDTA
Gene Name Synonym
Fibronectin leucine rich transmembrane protein 2
Subcellular Location
Extracellular matrix. Secreted. Endoplasmic reticulum membrane. Microsome membrane. Cell membrane. Single-pass membrane protein. Focal adhesion. Synaptosome.
Sample Data
Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.024 0.184 0.284 0.463 0.716 1.112 1.765 2.681 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.024 0.184 0.284 0.463 0.716 1.112 1.765 2.681 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.024 0.184 0.284 0.463 0.716 1.112 1.765 2.681 O.D. 0.024 0.184 0.284 0.463 0.716 1.112 1.765 2.681
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.

Related Products

CatalogName
EK1973Rat FLRT2 ELISA Kit PicoKine®
EK1971Human FLRT2 ELISA Kit PicoKine®
EK1971-carrier-freeHuman FLRT2 ELISA Kit PicoKine®, Carrier-free
EK1973-carrier-freeRat FLRT2 ELISA Kit PicoKine®, Carrier-free
EK1974Human FLRT3 ELISA Kit PicoKine®
EK2263Mouse MARCO ELISA Kit PicoKine®
EK2093Mouse IL4R ELISA Kit PicoKine®
EK2091Mouse CD99L2 ELISA Kit PicoKine®
EK1863Mouse SEZ6L ELISA Kit PicoKine®
EK2076Mouse GFRA3 ELISA Kit PicoKine®

Alternative Products

CatalogName
MBS1751714-01Mouse FLRT2 PicoKine ELISA Kit
EK1972Mouse FLRT2 ELISA Kit PicoKine®
EK1971Human FLRT2 ELISA Kit PicoKine®
MBS1751715-01Rat FLRT2 PicoKine ELISA Kit
MBS1751713-01Human FLRT2 PicoKine ELISA Kit
EK1973Rat FLRT2 ELISA Kit PicoKine®
CEK2280Mouse FLRT2 ELISA Kit
OKBB01346-96WFlrt2 ELISA Kit (Mouse)
OKBB01346Flrt2 ELISA Kit (Mouse) (OKBB01346)
OKEI00453FLRT2 ELISA Kit (Mouse) (OKEI00453)

Popular Products