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TMRM Perchlorate (solution)

CAT: 0804-HY-DY1042-01Size: 500 μLDry Ice: NoHazardous: No
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CAT#:0804-HY-DY1042-01Size:500 μL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms[1].Solvent and concentration: DMSO: 10 mM
CAS Number
115532-50-8
Target
Fluorescent Dye
Related Pathways
Others
Field of Research
Others
Smiles
O=C(C1=CC=CC=C1C2=C3C=CC(N(C)C)=CC3=[O+]C4=C2C=CC(N(C)C)=C4)OC.O=Cl(=O)([O-])=O
Molecular Formula
C25H25ClN2O7
Molecular Weight
500.93
References & Citations
[1]Crowley LC, et al. Measuring Mitochondrial Transmembrane Potential by TMRE Staining. Cold Spring Harb Protoc. 2016 Dec 1;2016 (12) :pdb.prot087361.|[2]Chowdhury SR, et al. Simultaneous evaluation of substrate-dependent oxygen consumption rates and mitochondrial membrane potential by TMRM and safranin in cortical mitochondria. Biosci Rep. 2015 Dec 8;36 (1) :e00286.|[3]Monteith A, et al. Imaging of mitochondrial and non-mitochondrial responses in cultured rat hippocampal neurons exposed to micromolar concentrations of TMRM. PLoS One. 2013;8 (3) :e58059.
Shipping Conditions
Blue Ice
Storage Conditions
Store at -20°C for 1 year, protect from light. Avoid repeated freezing and thawing
Scientific Category
Dye Reagents
Clinical Information
No Development Reported

Chemical Information

Tetramethylrhodamine Methyl Ester Perchlorate
CAS Number115532-50-8
PubChem CID11755725
IUPAC Name[6-(dimethylamino)-9-(2-methoxycarbonylphenyl)xanthen-3-ylidene]-dimethylazanium perchlorate
Molecular FormulaC25H25ClN2O7
Molecular Weight500.9
Topological Polar Surface Area116
Hydrogen Bond Donor Count0
Hydrogen Bond Acceptor Count8
Rotatable Bond Count4
Heavy Atom Count35
Formal Charge0
Complexity868
SMILES
CN(C)C1=CC2=C(C=C1)C(=C3C=CC(=[N+](C)C)C=C3O2)C4=CC=CC=C4C(=O)OC.[O-]Cl(=O)(=O)=O
InChI
InChI=1S/C25H25N2O3.ClHO4/c1-26(2)16-10-12-20-22(14-16)30-23-15-17(27(3)4)11-13-21(23)24(20)18-8-6-7-9-19(18)25(28)29-5;2-1(3,4)5/h6-15H,1-5H3;(H,2,3,4,5)/q+1;/p-1
InChIKey
PFYWPQMAWCYNGW-UHFFFAOYSA-M
Chemical Structure
2D Structure
2D structure of Tetramethylrhodamine Methyl Ester Perchlorate
CAS: 115532-50-8
CID: 11755725
Formula: C25H25ClN2O7
MW: 500.9
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight500.9
Hydrogen Bond Donor Count0
Hydrogen Bond Acceptor Count8
Rotatable Bond Count4
Exact Mass500.1350288
Monoisotopic Mass500.1350288
Topological Polar Surface Area116 Ų
Heavy Atom Count35
Formal Charge0
Complexity868
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count2
Compound Is CanonicalizedYes

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