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Recombinant human MIF protein

CAT: 0112-MIF0501-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-MIF0501-100Size:100 µg
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Description
The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. Recombinant human MIF, fused to His-tag at N-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.
Product Name Alternative
Macrophage migration inhibitory factor, Macrophage migration inhibitory factor, GLIF, MMIF, MIF (1-114aa), MIF, EC 5.3.2.1, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, Macrophage migration inhibitory factor Glycosylation inhibiting factor, MIF protein
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
17 kDa (155aa) confirmed by MALDI-TOF
Additionnal Information
MIF, Macrophage migration inhibitory factor, Macrophage migration inhibitory factor, GLIF, MMIF, MIF (1-114aa), MIF, EC 5.3.2.1, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, Macrophage migration inhibitory factor Glycosylation inhibiting factor, MIF protein, MIF0501-10 µg, MIF0501-20 µg, MIF0501-50 µg, MIF0501-100 µg, MIF0501-250 µg, MIF0501-500 µg, MIF0501-1 mg, MIF0501-10, MIF0501-20, MIF0501-50, MIF0501-100, MIF0501-250, MIF0501-500, MIF0501-1
References & Citations
Weiser WY., et al (1989) Proc Natl Acad Sci. 86: 7522-26.; ; Bernhagen J., et al (1994) Biochemistry. 33: 14144-55.; ; Bucala R., et al. (1996) FASEB J 10: 1607-13.;
Other References
Tsuji S, et al. Effective isolation of RNA aptamer through suppression of PCR bias. (Biochem Biophys Res Commun. 2009) {https://pubmed.ncbi.nlm.nih.gov/19520057/}; ; Tsuji S, et al. RNA aptamer binding to polyhistidine-tag. (Biochem Biophys Res Commun. 2009) {https://pubmed.ncbi.nlm.nih.gov/19520059/}; ; Garai J, et al. Tetralone derivatives are MIF tautomerase inhibitors and attenuate macrophage activation and amplify the hypothermic response in endotoxemic mice. (J Enzyme Inhib Med Chem. 2021) {https://pubmed.ncbi.nlm.nih.gov/34225560/}; ; Garai J, et al. Synthesis and evaluation of a new class of MIF-inhibitors in activated macrophage cells and in experimental septic shock in mice. (Eur J Med Chem. 2023) {https://pubmed.ncbi.nlm.nih.gov/36587420/}
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_002406.1
Uniprot Accession Number
P14174
Species
Human
AA Sequence
MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA

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