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Human MIF antibody

CAT: 0112-AMF0608-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-AMF0608-100Size:100 µL
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Description
The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.
Product Name Alternative
Macrophage migration inhibitory factor, GLIF, MMIF, MIF, EC 5.3.2.1, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, Macrophage migration inhibitory factor, macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human MIF (1-115 aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
40000
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
MIF, AMF0608-10 µg, AMF0608-20 µg, AMF0608-50 µg, AMF0608-100 µg, AMF0608-250 µg, AMF0608-500 µg, AMF0608-1 mg, AMF0608-10, AMF0608-20, AMF0608-50, AMF0608-100, AMF0608-250, AMF0608-500, AMF0608-1
References & Citations
Weiser WY, et al., (1989) Proc Natl Acad Sci. 86:7522-7526.; ; Bernhagen J, et al., (1994) Biochemistry. 33:14144-14155.; ; Richard B, et al., (1996) FASEB J. 10:1607-1613.
Other References
Koizumi M, et al. Positive correlations of age and parity with plasma concentration of macrophage migration inhibitory factor in Japanese black cows. (J Reprod Dev. 2016) {https://pubmed.ncbi.nlm.nih.gov/26853787/}
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Immunology
NCBI Accession Number
NP_002406
Uniprot Accession Number
P14174
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; Lane 2.: THP-1 cell lysate; ; Lane 3.: HeLa cell lysate; ; Lane 4.: U937 cell lysate The cell lysates of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1,000) . Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. {AMF0608-WB2.jpg}
IF Description
ICC/IF analysis of MIF in Balb/3T3 cells. The cell was stained with AMF0608 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of MIF in HeLa cells. The cell was stained with AMF0608 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{AMF0608-IF2.jpg}

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