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Recombinant E.coli G6PD protein

CAT: 0112-GPD0905-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-GPD0905-020Size:20 µg
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Description
Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia. Recombinant G6PD protein was expressed in E. coli and purified by conventional chromatography techniques.
Specifications
Specific activity is > 50 units/mg obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.
Product Name Alternative
Glucose-6-phosphate 1-dehydrogenase, NADP (+) -dependent glucose-6-phosphate dehydrogenase, zwf, b1852, JW1841
Expression System
E.coli
Antigen Species
E.coli
Tag
Non-Tagged
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is > 50unit/mg obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.
Molecular Weight
55.7 kDa (491aa) confirmed by MALDI-TOF
Additionnal Information
G6PD, Glucose-6-phosphate 1-dehydrogenase, Glucose-6-phosphate 1-dehydrogenase, zwf, Glucose-6-phosphate 1-dehydrogenase G6PD, G6PD1, G6pdx, Glucose 6 phosphate 1 dehydrogenase, MET19, POS10, Zwf1p., GPD0905-10 µg, GPD0905-20 µg, GPD0905-50 µg, GPD0905-100 µg, GPD0905-250 µg, GPD0905-500 µg, GPD0905-1 mg, GPD0905-10, GPD0905-20, GPD0905-50, GPD0905-100, GPD0905-250, GPD0905-500, GPD0905-1
References & Citations
Huang Y., et al. (2008) Mol Genet Metab. 93 (1) :44-53.; ; Zimny A., et al. (2003) Pol Arch Med Wewn. 110 (5) :1327-33;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 0.1mM PMSF, 2mM EDTA, 0.5mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_416366.1
Uniprot Accession Number
P0AC53
Species
E.coli
AA Sequence
MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E

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