Human GAPDH antibody

• Description:

  Multiple roles for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) have been recently appreciated. GAPDH is found in the particulate fractions, such as the nucleus, the mitochondria, and the small vesicular fractions. GAPDH gene expression is specifically increased during programmed neuronal cell death. When cells are exposed to various stressors, dynamic subcellular re-distribution of GAPDH occurs. GAPDH is also involved in various diseases, especially neurodegenerative disorders and cancers. As a membrane protein, GAPDH functions in endocytosis; in the cytoplasm, it is involved in the translational control of gene expression; in the nucleus, it functions in nuclear tRNA export, in DNA replication, and in DNA repair.

• Product Name Alternative:

  Glyceraldehyde-3-phosphate dehydrogenase isoform 1, Peptidyl-cysteine S-nitrosylase GAPDH, GAPD, G3PD

• Host:

  Mouse

• Antigen Species:

  Human

• Reactivity:

  Human

• Immunogen:

  Recombinant human GAPDH (1-335aa) purified from E.coli

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  AT8G4

• Conjugation:

  Unconjugated

• Applications:

  ELISA, WB, ICC/IF

• Purification Method:

  By protein-G affinity chromatography

• Concentration:

  1 mg/mL (determined by BCA assay)

• Additionnal Information:

  Wu:fb33a10, Peptidyl-cysteine S-nitrosylase GAPDH, Peptidyl cysteine S nitrosylase GAPDH, p38 component, OTTHuMP00000174432, OTTHuMP00000174431, OCT1 coactivator in S phase, OCAS, MGC88685, MGC127711, MGC105239, MGC103191, MGC103190, MGC102546, MGC102544, KNC-NDS6, KNC NDS6, HEL-S-162eP, HEL S 162eP, Glyceraldehyde-3-phosphate dehydrogenase isoform 2, Glyceraldehyde-3-phosphate dehydrogenase isoform 1, Glyceraldehyde-3-phosphate dehydrogenase 38 kDa BFA-dependent ADP-ribosylation substrate, Glyceraldehyde-3-phosphate dehydrogenase, Glyceraldehyde3 phosphate dehydrogenase, Glyceraldehyde 3 phosphate dehydrogenase isoform 2, Glyceraldehyde 3 phosphate dehydrogenase isoform 1, Glyceraldehyde 3 phosphate dehydrogenase 38 kDa BFA dependent ADP ribosylation substrate, Glyceraldehyde 3 phosphate dehydrogenase, GAPDH human, GAPDH, GAPD, G3PDH, G3PD, EC1.2.1.12, EC 1.2.1.12, cb609, BARS-38, BARS 38, Aging-associated gene 9 protein, Aging associated gene 9 protein, 38-KD component, 38 KD compone

• References & Citations:

  Chuang DM, et al. (2005) Annu Rev Pharmacol Toxicol, 45:269-90.; ; Mazzola JL, et al. (2002) Neurotoxicology, 23 (4-5) :603-9.; ; Sirover MA. (1997) J Cell Biochem, 66 (2) :133-40.

• Other References:

  Migas AA, et al. RUNX1T1/MTG8/ETO gene expression status in human t (8;21) (q22; q22) -positive acute myeloid leukemia cells. (Leuk Res. 2014) {https://pubmed.ncbi.nlm.nih.gov/24976338/}; ; Yu H, et al. Sulfasalazine‑induced ferroptosis in breast cancer cells is reduced by the inhibitory effect of estrogen receptor on the transferrin receptor. (Oncol Rep. 2019) {https://pubmed.ncbi.nlm.nih.gov/31173262/}

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

• Applications Notes:

  The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

• Scientific Category:

  Cancer

• NCBI Accession Number:

  NP_002037

• Uniprot Accession Number:

  P04406

• WB Description:

  The HeLa cell lysates (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GAPDH antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Anti-human GAPDH antibody (1:1000) ; ; Lane 2.: Anti-human GAPDH antibody (1:2000) ; ; Lane 3.: Anti-human GAPDH antibody (1:4000) ; ; Lane 4.: Anti-human GAPDH antibody (1:6000) ; ; Lane 5.: Anti-human GAPDH antibody (1:8000) ; ; Lane 6.: Anti-human GAPDH antibody (1:10000) ;

• IF Description:

  Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human GAPDH antibody (1:500) with Texas Red