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Recombinant human G-CSF protein

CAT: 0112-CSF4001-010Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0112-CSF4001-010Size:10 µg
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Description
Human granulocyte colony-stimulating factor (hG-CSF) is one of the hematopoietic growth factors which plays an important role in stimulating proliferation, differentiation, and functional activation of blood cells. In addition to its effect on hematopoiesis, G-CSF also enhances neutrophil functions against bacteria, fungi, and tumor cells mediated by antibody-dependent cell-mediated cytotoxicity. Recombinant hG-CSF was expressed in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Specifications
Human G-CSF stimulates cell proliferation of the M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤0.3 ng/mL.
Product Name Alternative
Granulocyte-colony stimulating factor, CSF3, GCSF, G-CSF, G-CSF, Pluripoietin, Filgrastim, Lenograstim, Granulocyte colony-stimulating factor, Granulocyte-colony stimulating factor Colony stimulating factor 3 (granulocyte), CSF 3, CSF beta, Csfg, GCSA, Granulocyte colony stimulating factor, Lenograstim, Macrophage granulocyte inducer 2, MGC45931, MGI 2
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤0.3 ng/mL.
Molecular Weight
18.8 kDa (175aa) confirmed by MALDI-TOF
Additionnal Information
G-CSF, CSF4001-10 µg, CSF4001-20 µg, CSF4001-50 µg, CSF4001-100 µg, CSF4001-250 µg, CSF4001-500 µg, CSF4001-1 mg, CSF4001-10, CSF4001-20, CSF4001-50, CSF4001-100, CSF4001-250, CSF4001-500, CSF4001-1
References & Citations
Nomura H, et al. (1986) EMBO J , 5 (5) : 871-6; ; Nagata, S., et al. (1986) Nature, 319 : 415-418; ; Demetri, G. D., et al. (1991) Blood, 78: 2791-2808 ; ; Hill, C. P., et al (1993) Proc. Natl. Acad. Sci. uSA, 90:5167-5171;
Other References
Lee EN, et al. Stabilizing peptide fusion for solving the stability and solubility problems of therapeutic proteins. Pharm Res. 2005 Oct;22 (10) :1735-46.{https://pubmed.ncbi.nlm.nih.gov/16180132/}
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_757373.1
Uniprot Accession Number
P09919
Species
Human
AA Sequence
MTPLGPASSL PQSFLLKCLE QVRKIQGDGA ALQEKLCATY KLCHPEELVL LGHSLGIPWA PLSSCPSQAL QLAGCLSQLH SGLFLYQGLL QALEGISPEL GPTLDTLQLD VADFATTIWQ QMEELGMAPA LQPTQGAMPA FASAFQRRAG GVLVASHLQS FLEVSYRVLR HLAQP

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