Recombinant human FGF basic/FGF-2 protein

• Description:

  Fibroblast growth factor 2 (FGF2) is a member of the FGF family that binds heparin and plays important roles in morphogenic, neurotrophic and angiogenic processes. FGF2 possess diverse biological functions, such as neuron differentiation, embryonic development and differentiation, modulation of angiogenesis, and wound healing. Recombinant human FGF2 was expressed in E. coli and purified by conventional chromatography techniques.

• Specifications:

  Human FGF-2 stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cells in the presence of 10 µg/mL of heparin. The ED50 range ≤ to 0.2 ng/mL.

• Product Name Alternative:

  Fibroblast growth factor 2, BFGF, FGFB, HBGF-2, FGF2, FGF-2

• Expression System:

  E.coli

• Antigen Species:

  Human

• Tag:

  Non-Tagged

• Applications:

  SDS-PAGE, Bioactivity

• Concentration:

  1 mg/mL (determined by Bradford assay)

• Endotoxin:

  < 1 EU per 1ug of protein (determined by LAL method)

• Purity:

  > 95% by SDS-PAGE

• Bioactivity:

  Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells in the presence of 10 µg/mL of heparin. The ED50 range ≤ 0.2 ng/mL.

• Molecular Weight:

  16.5 kDa (147aa) confirmed by MALDI-TOF

• Additionnal Information:

  FGF basic, Fibroblast growth factor 2, BFGF, FGFB, HBGF-2, ATGP4117-10 µg, ATGP4117-20 µg, ATGP4117-50 µg, ATGP4117-100 µg, ATGP4117-250 µg, ATGP4117-500 µg, ATGP4117-1 mg, ATGP4117-10, ATGP4117-20, ATGP4117-50, ATGP4117-100, ATGP4117-250, ATGP4117-500, ATGP4117-1

• References & Citations:

  Nakayama T., et al. (2007) Blood. 109 (2) :1363-72. ; ; A E Eriksson., et al. (1991) PNAS. 88:3441-3445; ; Raines, E.W. et al. (1985) Methods Enzymol. 109:749-73

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. 50 mM Tris-HCl buffer (pH 8.0)

• Scientific Category:

  Hormones & Cytokines

• NCBI Accession Number:

  NP_001997.5

• Uniprot Accession Number:

  P09038

• Species:

  Human

• AA Sequence:

  MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS