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Recombinant human CD4 protein

CAT: 0112-ATGP4108-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP4108-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD4, also known as T-cell surface antigen T4/Leu-3, is a member of the immunoglobulin superfamily. It is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. It interacts directly with MHC class II molecules on the surface of the antigen presenting cell via its extracellular domain. It is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen presenting cell. Also, it binds directly to MHC class II molecules on antigen presenting cells. This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. Recombinant human CD4, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Specifications
Human CD4 stimulates cell adhesion of the HeLa human cervical epithelial carcinoma cells. The ED50 range ≤ 10 µg/mL.
Product Name Alternative
T-cell surface glycoprotein CD4, CD4, CD4mut, T-cell surface glycoprotein CD4 isoform 1, T-cell surface antigen T4/Leu-3, OKT4D
Expression System
HEK293
Antigen Species
Human
Tag
HIgG-His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.25 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Purity
90% by SDS - PAGE
Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells. When cells are added to human CD4 coated plates. The ED50 range ≤ 10 µg/mL.
Molecular Weight
67.7kDa (604aa)
Additionnal Information
ATGP4108-10 µg, ATGP4108-20 µg, ATGP4108-50 µg, ATGP4108-100 µg, ATGP4108-250 µg, ATGP4108-500 µg, ATGP4108-1 mg, ATGP4108-10, ATGP4108-20, ATGP4108-50, ATGP4108-100, ATGP4108-250, ATGP4108-500, ATGP4108-1
References & Citations
Singh SK., et al. (2012) FEBS J. 279:3705-3714.; ; Farrar WL., et al. (1988) Crit Rev Immunol. 8:315-339.; ; Doyle, C. and J.L. Strominger (1987) Nature 330:256-259.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 40% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_000607.1
Uniprot Accession Number
P01730
Species
Human
N Terminal Sequence
Lys26

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