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Recombinant rat ACE-2 protein

CAT: 0112-ATGP4048-010Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP4048-010Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ACE-2, also known as angiotensin-converting enzyme 2, is carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. It is able to hydrolyze apelin-13 and dynorphin-13 with high efficiency and may be an important regulator of heart function. Recombinant rat ACE-2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Specifications
Specific activity is > 150 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of Mca-YVADAPK (Dnp) -OH per minute at pH 7.5, at 25C.
Product Name Alternative
ACE2, angiotensin-converting enzyme2, Ace2, ACE-related carboxypeptidase
Expression System
Baculovirus
Antigen Species
Rat
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is > 150 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of Mca-YVADAPK (Dnp) -OH per minute at pH 7.5, at 25C.
Molecular Weight
84.7 kDa (731aa)
Additionnal Information
ATGP4048-10 µg, ATGP4048-20 µg, ATGP4048-50 µg, ATGP4048-100 µg, ATGP4048-250 µg, ATGP4048-500 µg, ATGP4048-1 mg, ATGP4048-10, ATGP4048-20, ATGP4048-50, ATGP4048-100, ATGP4048-250, ATGP4048-500, ATGP4048-1
References & Citations
Li W., et al. (2004) J. Virol. 78:11429-11433.; ; Crackower M., A. et al. (2002) Nature 417:822-828.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Enzymes & Proteases, Virus
NCBI Accession Number
NP_001012006.1
Uniprot Accession Number
Q5EGZ1
Species
Rat
N Terminal Sequence
Gln18

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