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Recombinant human CNTF protein

CAT: 0112-ATGP3455-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP3455-020Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CNTF (Ciliary neurotrophic factor) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. This protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. CNTF protein is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM) . It is localized in the cell nucleus subsequent to receptor binding. Recombinant human CNTF, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Specifications
Human CNTF in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 1 µg/mL.
Product Name Alternative
Ciliary neurotrophic factor
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 1 µg/mL.
Molecular Weight
25 kDa (220aa) confirmed by MALDI-TOF
Additionnal Information
CNTF, Ciliary neurotrophic factor, ATGP3455-10 µg, ATGP3455-20 µg, ATGP3455-50 µg, ATGP3455-100 µg, ATGP3455-250 µg, ATGP3455-500 µg, ATGP3455-1 mg, ATGP3455-10, ATGP3455-20, ATGP3455-50, ATGP3455-100, ATGP3455-250, ATGP3455-500, ATGP3455-1
References & Citations
Sendther M., et al. (1994), J Neurobiol. 25 (11) :1436-53.; ; Robert F., et al. (2007), J. Biol. Chem. 282 (46) : 33421-33434
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 1mM DTT, 30% glycerol, 0.2M NaCl
Scientific Category
Neuroscience
NCBI Accession Number
NP_000605
Uniprot Accession Number
P26441
Species
Human
AA Sequence
MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM

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