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Recombinant mouse PGAM1 protein

CAT: 0112-ATGP3427-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP3427-020Size:20 µg
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Description
PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2, 3-BPGA (2, 3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB) . Recombinant mouse PGAM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Specifications
Specific activity is >150units/mg, in which One unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.
Product Name Alternative
Phosphoglycerate mutase 1, 2310050F24Rik, Pgam-1
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is >150unit/mg, in which one unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.
Molecular Weight
31.4 kDa (278aa) confirmed by MALDI-TOF
Additionnal Information
PGAM1, Phosphoglycerate mutase 1, 2310050F24Rik, Pgam-1, ATGP3427-10 µg, ATGP3427-20 µg, ATGP3427-50 µg, ATGP3427-100 µg, ATGP3427-250 µg, ATGP3427-500 µg, ATGP3427-1 mg, ATGP3427-10, ATGP3427-20, ATGP3427-50, ATGP3427-100, ATGP3427-250, ATGP3427-500, ATGP3427-1
References & Citations
Cao L., et al. (2007) J. Immunol. 179:5864-5876; ; Ballif B.A., et al. (2008) J. Proteome Res. 7:311-318
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Cancer
NCBI Accession Number
NP_075907
Uniprot Accession Number
Q9DBJ1
Species
Mouse
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAAYKL VLIRHGESAW NLENRFSGWY DADLSPAGHE EAKRGGQALR DAGYEFDICF TSVQKRAIRT LWTVLDAIDQ MWLPVVRTWR LNERHYGGLT GLNKAETAAK HGEAQVKIWR RSYDVPPPPM EPDHPFYSNI SKDRRYADLT EDQLPSCESL KDTIARALPF WNEEIVPQIK EGKRVLIAAH GNSLRGIVKH LEGLSEEAIM ELNLPTGIPI VYELDKNLKP IKPMQFLGDE ETVRKAMEAV AAQGKVKK

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