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Human PGAM1 antibody

CAT: 0112-ATGA0352-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0352-100Size:100 µL
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Description
PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2, 3-BPGA (2, 3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB) . Mutations in this protein cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X.
Product Name Alternative
Phosphoglycerate mutase 1, Phosphoglycerate mutase isozyme B, PGAM-B, PGAMA, Phosphoglycerate mutase 1
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human PGAM1 (1-254aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a λ
Clone
AT1G4
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
PGAM1, ATGA0352-10 µg, ATGA0352-20 µg, ATGA0352-50 µg, ATGA0352-100 µg, ATGA0352-250 µg, ATGA0352-500 µg, ATGA0352-1 mg, ATGA0352-10, ATGA0352-20, ATGA0352-50, ATGA0352-100, ATGA0352-250, ATGA0352-500, ATGA0352-1
References & Citations
Vander-Heiden MG., et al. (2010) Science. 329: 1492-1499.; ; Jacobowitz DM., et al. (2008) Microvasc Res. 76: 89-93.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Metabolism
NCBI Accession Number
NP_002620
Uniprot Accession Number
P18669
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGAM1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : 293T cell lysate; ; Lane 2. : Jurkat cell lysate; ; Lane 3. : Raji cell lysate; ; Lane 4. : A431 cell lysate; ; Lane 5. : HeLa cell lysate
IF Description
ICC/IF analysis of PGAM1 in HeLa cells. The cell was stained with ATGA0352 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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