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Recombinant e.coli glpK protein

CAT: 0112-ATGP3062-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP3062-500Size:500 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
GlpK also known as glycerol kinase, belongs to the FGGY kinase family. glpK function to catalyze the transfer of a phosphate group from ATP to glycerol, thereby forming glycerol phosphate. This intermediate can then be converted to dihydroxyacetone phosphate (DHAP), which is utilized in either glycolysis or gluconeogenesis. Recombinant E. coli glpK, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Glycerol kinase
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
58.6 kDa (525aa)
Additionnal Information
GlpK, Glycerol kinase, ATGP3062-10 µg, ATGP3062-20 µg, ATGP3062-50 µg, ATGP3062-100 µg, ATGP3062-250 µg, ATGP3062-500 µg, ATGP3062-1 mg, ATGP3062-10, ATGP3062-20, ATGP3062-50, ATGP3062-100, ATGP3062-250, ATGP3062-500, ATGP3062-1
References & Citations
Freddolino, P.L., et al. (2012) J. Bacteriol. 194 (2), 303-306
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT
Scientific Category
Cancer
NCBI Accession Number
NP_418361
Uniprot Accession Number
P0A6F3
Species
E.coli
AA Sequence
MTEKKYI VALDQGTTSS RAVVMDHDAN IISVSQREFE QIYPKPGWVE HDPMEIWATQ SSTLVEVLAK ADISSDQIAA IGITNQRETT IVWEKETGKP IYNAIVWQCR RTAEICEHLK RDGLEDYIRS NTGLVIDPYF SGTKVKWILD HVEGSRERAR RGELLFGTVD TWLIWKMTQG RVHVTDYTNA SRTMLFNIHT LDWDDKMLEV LDIPREMLPE VRRSSEVYGQ TNIGGKGGTR IPISGIAGDQ QAALFGQLCV KEGMAKNTYG TGCFMLMNTG EKAVKSENGL LTTIACGPTG EVNYALEGAV FMAGASIQWL RDEMKLINDA YDSEYFATKV QNTNGVYVVP AFTGLGAPYW DPYARGAIFG LTRGVNANHI IRATLESIAY QTRDVLEAMQ ADSGIRLHAL RVDGGAVANN FLMQFQSDIL GTRVERPEVR EVTALGAAYL AGLAVGFWQN LDELQEKAVI EREFRPGIET TERNYRYAGW KKAVKRAMAW EEHDE

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