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Recombinant human IMPACT protein

CAT: 0112-ATGP2266-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP2266-020Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
IMPACT belongs to the IMPACT family and contains 1 RWD domain. This protein is translational regulator that ensures constant high levels of translation under amino acid starvation and acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis. Recombinant human IMPACT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Protein IMPACT, RWDD5
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
38.9 kDa (343aa) confirmed by MALDI-TOF
Additionnal Information
IMPACT, protein IMPACT, RWDD5, ATGP2266-10 µg, ATGP2266-20 µg, ATGP2266-50 µg, ATGP2266-100 µg, ATGP2266-250 µg, ATGP2266-500 µg, ATGP2266-1 mg, ATGP2266-10, ATGP2266-20, ATGP2266-50, ATGP2266-100, ATGP2266-250, ATGP2266-500, ATGP2266-1
References & Citations
Habibi, D., et al. (2010) J. Cell. Physiol. 225 (1), 196-205; ; Pereira, C.M., et al. (2005) J. Biol. Chem. 280 (31), 28316-28323
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_060909
Uniprot Accession Number
Q9P2X3
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAEGDAG SDQRQNEEIE AMAAIYGEEW CVIDDCAKIF CIRISDDIDD PKWTLCLQVM LPNEYPGTAP PIYQLNAPWL KGQERADLSN SLEEIYIQNI GESILYLWVE KIRDVLIQKS QMTEPGPDVK KKTEEEDVEC EDDLILACQP ESSVKALDFD ISETRTEVEV EELPPIDHGI PITDRRSTFQ AHLAPVVCPK QVKMVLSKLY ENKKIASATH NIYAYRIYCE DKQTFLQDCE DDGETAAGGR LLHLMEILNV KNVMVVVSRW YGGILLGPDR FKHINNCARN ILVEKNYTNS PEESSKALGK NKKVRKDKKR NEH

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