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Recombinant human CHMP1B protein

CAT: 0112-ATGP1571-250Size: 250 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1571-250Size:250 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CHMP1B, also known as charged multivesicular body protein 1b, is probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Recombinant human CHMP1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Name Alternative
Charged multivesicular body protein 1B, CHMP1.5, Chromatin-modifying protein 1b, CHMP1b, Vacuolar protein sorting-associated protein 46-2, Vps46-2, hVps46-2, C18orf2, Vps46B
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
24.7 kDa (223aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
CHMP1B, Charged multivesicular body protein 1b, C10orf2, C18-ORF2, C18orf2, CHMP1.5, hVps46-2, Vps46-2, Vps46B, ATGP1571-10 µg, ATGP1571-20 µg, ATGP1571-50 µg, ATGP1571-100 µg, ATGP1571-250 µg, ATGP1571-500 µg, ATGP1571-1 mg, ATGP1571-10, ATGP1571-20, ATGP1571-50, ATGP1571-100, ATGP1571-250, ATGP1571-500, ATGP1571-1
References & Citations
Martin-Serrano J., et al. (2003) Proc. Natl. Acad. Sci. u.S.A. 100:12414-12419; ; Bajorek M., et al. (2009) Mol. Biol. Cell. 20:1360-1373
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Cell Cycle
NCBI Accession Number
NP_065145.2
Uniprot Accession Number
Q7LBR1
Species
Human
AA Sequence
MSNMEK HLFNLKFAAK ELSRSAKKCD KEEKAEKAKI KKAIQKGNME VARIHAENAI RQKNQAVNFL RMSARVDAVA ARVQTAVTMG KVTKSMAGVV KSMDATLKTM NLEKISALMD KFEHQFETLD VQTQQMEDTM SSTTTLTTPQ NQVDMLLQEM ADEAGLDLNM ELPQGQTGSV GTSVASAEQD ELSQRLARLR DQV

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