Recombinant e.coli mutM protein

• Description :

  MutM, also known as formamidopyrimidine DNA glycosylase, is a base excision repair enzyme which recognizes and removes a wide range of oxidized purines from correspondingly damaged DNA. This protein is nonredundant and required to rapidly remove its substrate lesions on the chromosome. In addition, it also repaired a significant portion of the lesions recognized by Endo III, suggesting that it plays a prominent role in the global repair of both purine damage and pyrimidine damage in vivo. Recombinant E. coli mutM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

• Product Name Alternative :

  Formamidopyrimidine-DNA glycosylase, fpg

• Expression System :

  E.coli

• Antigen Species :

  E.coli

• Tag :

  His-Tag

• Applications :

  SDS-PAGE

• Concentration :

  0.5 mg/mL (determined by Bradford assay)

• Purity :

  > 90% by SDS-PAGE

• Molecular Weight :

  32.4 kDa (289aa) confirmed by MALDI-TOF

• Additionnal Information :

  MutM, ATGP1448-10 µg, ATGP1448-20 µg, ATGP1448-50 µg, ATGP1448-100 µg, ATGP1448-250 µg, ATGP1448-500 µg, ATGP1448-1 mg, ATGP1448-10, ATGP1448-20, ATGP1448-50, ATGP1448-100, ATGP1448-250, ATGP1448-500, ATGP1448-1

• References & Citations :

  Serre L. et al. (2002) EMBO J. 21:2854-2865; ; Schalow BJ. et al. (2011) J Mol Biol, 410:183-193;

• Storage Conditions :

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation :

  Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1M NaCl,1mM DTT

• Scientific Category :

  Enzymes & Proteases

• NCBI Accession Number :

  NP_418092

• Uniprot Accession Number :

  P05523

• Species :

  E.coli

• AA Sequence :

  MPELPEVETS RRGIEPHLVG ATILHAVVRN GRLRWPVSEE IYRLSDQPVL SVQRRAKYLL LELPEGWIII HLGMSGSLRI LPEELPPEKH DHVDLVMSNG KVLRYTDPRR FGAWLWTKEL EGHNVLTHLG PEPLSDDFNG EYLHQKCAKK KTAIKPWLMD NKLVVGVGNI YASESLFAAG IHPDRLASSL SLAECELLAR VIKAVLLRSI EQGGTTLKDF LQSDGKPGYF AQELQVYGRK GEPCRVCGTP IVATKHAQRA TFYCRQCQK