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Recombinant e.coli mutM protein

CAT: 0112-ATGP1448-010Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1448-010Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
MutM, also known as formamidopyrimidine DNA glycosylase, is a base excision repair enzyme which recognizes and removes a wide range of oxidized purines from correspondingly damaged DNA. This protein is nonredundant and required to rapidly remove its substrate lesions on the chromosome. In addition, it also repaired a significant portion of the lesions recognized by Endo III, suggesting that it plays a prominent role in the global repair of both purine damage and pyrimidine damage in vivo. Recombinant E. coli mutM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Formamidopyrimidine-DNA glycosylase, fpg
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
32.4 kDa (289aa) confirmed by MALDI-TOF
Additionnal Information
MutM, ATGP1448-10 µg, ATGP1448-20 µg, ATGP1448-50 µg, ATGP1448-100 µg, ATGP1448-250 µg, ATGP1448-500 µg, ATGP1448-1 mg, ATGP1448-10, ATGP1448-20, ATGP1448-50, ATGP1448-100, ATGP1448-250, ATGP1448-500, ATGP1448-1
References & Citations
Serre L. et al. (2002) EMBO J. 21:2854-2865; ; Schalow BJ. et al. (2011) J Mol Biol, 410:183-193;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1M NaCl,1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_418092
Uniprot Accession Number
P05523
Species
E.coli
AA Sequence
MPELPEVETS RRGIEPHLVG ATILHAVVRN GRLRWPVSEE IYRLSDQPVL SVQRRAKYLL LELPEGWIII HLGMSGSLRI LPEELPPEKH DHVDLVMSNG KVLRYTDPRR FGAWLWTKEL EGHNVLTHLG PEPLSDDFNG EYLHQKCAKK KTAIKPWLMD NKLVVGVGNI YASESLFAAG IHPDRLASSL SLAECELLAR VIKAVLLRSI EQGGTTLKDF LQSDGKPGYF AQELQVYGRK GEPCRVCGTP IVATKHAQRA TFYCRQCQK