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Recombinant human BDH1 protein

CAT: 0112-ATGP1341-250Size: 250 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1341-250Size:250 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
BDH1 is a member of the short-chain dehydrogenase/reductase gene family. This protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. It catalyzes the interconversion of acetoacetate and (R) -3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Recombinant human BDH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Name Alternative
D-beta-hydroxybutyrate dehydrogenase, BDH, MGC2723, MGC4347, MGC9788, SDR9C1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
35.3 kDa (318aa) confirmed by MALDI-TOF
Additionnal Information
BDH1, D-beta-hydroxybutyrate dehydrogenase, BDH, MGC2723, MGC4347, MGC9788, SDR9C1, ATGP1341-10 µg, ATGP1341-20 µg, ATGP1341-50 µg, ATGP1341-100 µg, ATGP1341-250 µg, ATGP1341-500 µg, ATGP1341-1 mg, ATGP1341-10, ATGP1341-20, ATGP1341-50, ATGP1341-100, ATGP1341-250, ATGP1341-500, ATGP1341-1
References & Citations
Marks A.R. et al. (1992) J Biol Chem. 267:15459-15463.; ; Adami P. et al. (1993) Biochem. J. 292: 863-872.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol 0.2M NaCl,2mM DTT, 0.1mM PMSF
Scientific Category
Metabolism
NCBI Accession Number
NP_976059
Uniprot Accession Number
Q02338
Species
Human
AA Sequence
ASAAEPVGS KAVLVTGCDS GFGFSLAKHL HSKGFLVFAG CLMKDKGHDG VKELDSLNSD RLRTVQLNVC SSEEVEKVVE IVRSSLKDPE KGMWGLVNNA GISTFGEVEF TSLETYKQVA EVNLWGTVRM TKSFLPLIRR AKGRVVNISS MLGRMANPAR SPYCITKFGV EAFSDCLRYE MYPLGVKVSV VEPGNFIAAT SLYSPESIQA IAKKMWEELP EVVRKDYGKK YFDEKIAKME TYCSSGSTDT SPVIDAVTHA LTATTPYTRY HPMDYYWWLR MQIMTHLPGA ISDMIYIR

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