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Recombinant e.coli ansA protein

CAT: 0112-ATGP1077-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1077-500Size:500 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
AnsA is the cytoplasmic asparaginase from Escherichia coli involved in intracellular asparagine utilization. Escherichia coli possesse two l-asparaginases: the cytoplasmic type I form (ansA) and the periplasmic type II form (ansB) . AnsA (Type I asparaginase) is constitutively expressed and required for the growth of the bacteria on asparagine as the sole nitrogen source. Recombinant E. coli ansA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Cytoplasmic L-asparaginase I, ECK1765, JW1756
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
39.3 kDa (358aa) confirmed by MALDI-TOF
Additionnal Information
AnsA, Cytoplasmic L-asparaginase I, ECK1765, JW1756, ATGP1077-10 µg, ATGP1077-20 µg, ATGP1077-50 µg, ATGP1077-100 µg, ATGP1077-250 µg, ATGP1077-500 µg, ATGP1077-1 mg, ATGP1077-10, ATGP1077-20, ATGP1077-50, ATGP1077-100, ATGP1077-250, ATGP1077-500, ATGP1077-1
References & Citations
Cantor JR., et al. (2009) Biochemistry. 48 (46) :11026-31. ; ; Weidle uH., et al. (2009) Anticancer Res. 29 (4) :951-63.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_416281
Uniprot Accession Number
P0A962
Species
E.coli
AA Sequence
MQKKSIYVAY TGGTIGMQRS EQGYIPVSGH LQRQLALMPE FHRPEMPDFT IHEYTPLMDS SDMTPEDWQH IAEDIKAHYD DYDGFVILHG TDTMAYTASA LSFMLENLGK PVIVTGSQIP LAELRSDGQI NLLNALYVAA NYPINEVTLF FNNRLYRGNR TTKAHADGFD AFASPNLPPL LEAGIHIRRL NTPPAPHGEG ELIVHPITPQ PIGVVTIYPG ISADVVRNFL RQPVKALILR SYGVGNAPQN KAFLQELQEA SDRGIVVVNL TQCMSGKVNM GGYATGNALA HAGVIGGADM TVEATLTKLH YLLSQELDTE TIRKAMSQNL RGELTPDD

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