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Recombinant human SEPSECS protein

CAT: 0112-ATGP0806-050Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0806-050Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SEPSECS, also known as SLA/LP autoantigen, catalyzes the final step of sec synthesis by converting O-phosphoseryl-tRNA (sec) to selenocysteinyl-tRNA (sec) using selenophosphate as the selenium donor. Also, this protein is considered a specific marker of autoimmune hepatitis. Recombinant human SEPSECS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
O-phosphoseryl-tRNA (Sec) selenium transferase, Liver-pancreas antigen, LP, SLA-p35, SLA/LP autoantigen, Selenocysteine synthase, Sec synthase, Selenocysteinyl-tRNA (Sec) synthase, Sep-tRNA:Sec-tRNA synthase, SepSecS, Soluble liver antigen, SLA, UGA suppressor tRNA-associated protein, tRNA (Ser/Sec) -associated antigenic protein, TRNP48
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
57.9 kDa (521aa) confirmed by MALDI-TOF
Additionnal Information
Liver-pancreas antigen, LP, O-phosphoseryl-tRNA (Sec) selenium transferase, Sec synthase, Selenocysteine synthase, Selenocysteinyl-tRNA (Sec) synthase, SepSecS, Sep-tRNA:Sec-tRNA synthase, SLA, SLA/LP autoantigen, SLA-p35, Soluble liver antigen, tRNA (Ser/Sec) -associated antigenic protein, TRNP48, UGA suppressor tRNA-associated protein, ATGP0806-10 µg, ATGP0806-20 µg, ATGP0806-50 µg, ATGP0806-100 µg, ATGP0806-250 µg, ATGP0806-500 µg, ATGP0806-1 mg, ATGP0806-010, ATGP0806-020, ATGP0806-050, ATGP0806-100, ATGP0806-250, ATGP0806-500, ATGP0806-01M
References & Citations
Palioura S., et al. (2009) Science. 325 (5938) :321-5.; ; Yuan J., et al. (2006) Proc. Natl. Acad. Sci. u.S.A. 103:18923-18927
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 40% glycerol, 0.15M NaCl, 1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_058651.3
Uniprot Accession Number
Q9HD40
Species
Human
AA Sequence
MNRESFAAGE RLVSPAYVRQ GCEARRSHEH LIRLLLEKGK CPENGWDEST LELFLHELAI MDSNNFLGNC GVGEREGRVA SALVARRHYR FIHGIGRSGD ISAVQPKAAG SSLLNKITNS LVLDIIKLAG VHTVANCFVV PMATGMSLTL CFLTLRHKRP KAKYIIWPRI DQKSCFKSMI TAGFEPVVIE NVLEGDELRT DLKAVEAKVQ ELGPDCILCI HSTTSCFAPR VPDRLEELAV ICANYDIPHI VNNAYGVQSS KCMHLIQQGA RVGRIDAFVQ SLDKNFMVPV GGAIIAGFND SFIQEISKMY PGRASASPSL DVLITLLSLG SNGYKKLLKE RKEMFSYLSN QIKKLSEAYN ERLLHTPHNP ISLAMTLKTL DEHRDKAVTQ LGSMLFTRQV SGARVVPLGS MQTVSGYTFR GFMSHTNNYP CAYLNAASAI GMKMQDVDLF IKRLDRCLKA VRKERSKESD DNYDKTEDVD IEEMALKLDN VLLDTYQDAS S

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