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Recombinant mouse SEPSECS protein

CAT: 0112-ATGP3813-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP3813-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Sepsecs, also known as O-phosphoseryl-tRNA (Sec) selenium transferase, catalyzes the final step of sec synthesis by converting O-phosphoseryl-tRNA (sec) to selenocysteinyl-tRNA (sec) using selenophosphate as the selenium donor. Also, this protein is considered a specific marker of autoimmune hepatitis. Recombinant mouse Sepsecs protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
O-phosphoseryl-tRNA (Sec) selenium transferase, Selenocysteine synthase, Sec synthase, Selenocysteinyl-tRNA (Sec) synthase, Sep-tRNA:Sec-tRNA synthase, UGA suppressor tRNA-associated protein
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
57.7 kDa (527aa) confirmed by MALDI-TOF
Additionnal Information
O-phosphoseryl-tRNA (Sec) selenium transferase, Selenocysteine synthase, Sec synthase, Selenocysteinyl-tRNA (Sec) synthase, Sep-tRNA:Sec-tRNA synthase, UGA suppressor tRNA-associated protein, O-phosphoseryl-tRNA (Sec) selenium transferase, AA986712, D5Ertd135e, SecS, SLA, Sepsecs, O phosphoseryl tRNA (Sec) selenium transferase, Sep tRNA : Sec tRNA synthase, ATGP3813-10 µg, ATGP3813-20 µg, ATGP3813-50 µg, ATGP3813-100 µg, ATGP3813-250 µg, ATGP3813-500 µg, ATGP3813-1 mg, ATGP3813-010, ATGP3813-020, ATGP3813-050, ATGP3813-100, ATGP3813-250, ATGP3813-500, ATGP3813-01M
References & Citations
Palioura S., et al. (2009) Science. 325 (5938) :321-5.; ; Yuan J., et al. (2006) Proc. Natl Acad Sci U.S.A. 103 (50) :18923-7.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 0.2M NaCl, 50% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_766078
Uniprot Accession Number
Q6P6M7
Species
Mouse
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMNPESFA AGERRVSPAY VRQGCEARRA HEHLIRLLLE QGKCPEDGWD ESTLELFLHE LAVMDSNNFL GNCGVGEREG RVASALVARR HYRFIHGIGR SGDISAVQPK AAGSSLLNKI TNSLVLNVIK LAGVHSVASC FVVPMATGMS LTLCFLTLRH KRPKAKYIIW PRIDQKSCFK SMVTAGFEPV VIENVLEGDE LRTDLKAVEA KIQELGPEHI LCLHSTTACF APRVPDRLEE LAVICANYDI PHVVNNAYGL QSSKCMHLIQ QGARVGRIDA FVQSLDKNFM VPVGGAIIAG FNEPFIQDIS KMYPGRASAS PSLDVLITLL SLGCSGYRKL LKERKEMFVY LSTQLKKLAE AHNERLLQTP HNPISLAMTL KTIDGHHDKA VTQLGSMLFT RQVSGARAVP LGNVQTVSGH TFRGFMSHAD NYPCAYLNAA AAIGMKMQDV DLFIKRLDKC LNIVRKEQTR ASVVSGADRN KAEDADIEEM ALKLDDVLGD VGQGPAL

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