Recombinant human eIF-4E/EIF4E protein

CAT: 0112-ATGP0663-050Size: 50 µgDry Ice: NoHazardous: No
CAT#:0112-ATGP0663-050Size:50 µg
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Description
EIF4E, a member of eukaryotic initiation factor 4 families, modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. This protein recognizes and binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, thereby mediating the initiation of translation. It facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Recombinant human EIF4E protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Name Alternative
Eukaryotic translation initiation factor 4E, CBP, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
27.2 kDa (237aa) confirmed by MALDI-TOF
Additionnal Information
EIF4E, Eukaryotic translation initiation factor 4E, CBP, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, ATGP0663-10 µg, ATGP0663-20 µg, ATGP0663-50 µg, ATGP0663-100 µg, ATGP0663-250 µg, ATGP0663-500 µg, ATGP0663-1 mg, ATGP0663-10, ATGP0663-20, ATGP0663-50, ATGP0663-100, ATGP0663-250, ATGP0663-500, ATGP0663-1
References & Citations
Bendahmane A., et al. (2010) PLoS One. 5 (6) :e11313.; ; Pelletier J., et al. (2010) J Gen Virol. 91 (Pt 5) :1224-8.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_001959.1
Uniprot Accession Number
P06730
Species
Human
AA Sequence
MATVEPETTP TPNPPTTEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LETLLCLIGE SFDDYSDDVC GAVVNVRAKG DKIAIWTTEC ENREAVTHIG RVYKERLGLP PKIVIGYQSH ADTATKSGST TKNRFVV

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