Recombinant human eIF-4E/EIF4E protein

• Description:

  EIF4E, a member of eukaryotic initiation factor 4 families, modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. This protein recognizes and binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, thereby mediating the initiation of translation. It facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Recombinant human EIF4E protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

• Product Name Alternative:

  Eukaryotic translation initiation factor 4E, CBP, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein

• Expression System:

  E.coli

• Antigen Species:

  Human

• Tag:

  His-Tag

• Applications:

  SDS-PAGE

• Concentration:

  0.5 mg/mL (determined by Bradford assay)

• Purity:

  > 85% by SDS-PAGE

• Molecular Weight:

  27.2 kDa (237aa) confirmed by MALDI-TOF

• Additionnal Information:

  EIF4E, Eukaryotic translation initiation factor 4E, CBP, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, ATGP0663-10 µg, ATGP0663-20 µg, ATGP0663-50 µg, ATGP0663-100 µg, ATGP0663-250 µg, ATGP0663-500 µg, ATGP0663-1 mg, ATGP0663-10, ATGP0663-20, ATGP0663-50, ATGP0663-100, ATGP0663-250, ATGP0663-500, ATGP0663-1

• References & Citations:

  Bendahmane A., et al. (2010) PLoS One. 5 (6) :e11313.; ; Pelletier J., et al. (2010) J Gen Virol. 91 (Pt 5) :1224-8.;

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

• Scientific Category:

  Epigenomics (Transcription & Translation)

• NCBI Accession Number:

  NP_001959.1

• Uniprot Accession Number:

  P06730

• Species:

  Human

• AA Sequence:

  MATVEPETTP TPNPPTTEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LETLLCLIGE SFDDYSDDVC GAVVNVRAKG DKIAIWTTEC ENREAVTHIG RVYKERLGLP PKIVIGYQSH ADTATKSGST TKNRFVV