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Recombinant human PDCD6IP protein

CAT: 0112-ATGP0482-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0482-100Size:100 µg
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Description
Alix, also known as PDCD6IP, is a cytoplasmic protein that interacts with apoptosis-associated proteins (ALG-2 and PDCD6) and with the endocytosis-regulator CIN85. It's involved in concentration and sorting of cargo proteins of the multivesicular body for incorporation into intralumenal vesicles that are generated by invagination and scission from the limiting membrane of the endosome. Overexpression of this protein and endophilins results in cytoplasmic vacuolization which may be partly responsible for the protection against cell death. Recombinant PDCD6IP protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Programmed cell death 6 interacting protein, PDCD6-interacting protein, ALG-2 interacting protein X, ALG-2-interacting protein 1, Alix, AIP1, Hp95
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
45.8 kDa (412aa) confirmed by MALDI-TOF
Additionnal Information
Alix, ATGP0482-10 µg, ATGP0482-20 µg, ATGP0482-50 µg, ATGP0482-100 µg, ATGP0482-250 µg, ATGP0482-500 µg, ATGP0482-1 mg, ATGP0482-10, ATGP0482-20, ATGP0482-50, ATGP0482-100, ATGP0482-250, ATGP0482-500, ATGP0482-1
References & Citations
Morita E., et al. (2007) EMBO J. 26 (19) :4215-27. ; ; Katoh K., et al. (2004) Arch Biochem Biophys. 421 (1) :159-65.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT
Scientific Category
Apoptosis
NCBI Accession Number
NP_037506.2
Uniprot Accession Number
Q8WUM4
Species
Human
AA Sequence
MATFISVQLK KTSEVDLAKP LVKFIQQTYP SGGEEQAQYC RAAEELSKLR RAAVGRPLDK HEGALETLLR YYDQICSIEP KFPFSENQIC LTFTWKDAFD KGSLFGGSVK LALASLGYEK SCVLFNCAAL ASQIAAEQNL DNDEGLKIAA KHYQFASGAF LHIKETVLSA LSREPTVDIS PDTVGTLSLI MLAQAQEVFF LKATRDKMKD AIIAKLANQA ADYFGDAFKQ CQYKDTLPKE VFPVLAAKHC IMQANAEYHQ SILAKQQKKF GEEIARLQHA AELIKTVASR YDEYVNVKDF SDKINRALAA AKKDNDFIYH DRVPDLKDLD PIGKATLVKS TPVNVPISQK FTDLFEKMVP VSVQQSLAAY NQRKADLVNR SIAQMREATT LA

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