ALIX (PDCD6IP) (NM_013374) Human Recombinant Protein

• Background:

  This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012]

• Synonyms:

  AIP1; ALIX; DRIP4; HP95

• Gene ID:

  10015

• UniProt:

  Q8WUM4

• Accession Number mRNA:

  NM_013374

• Chromosomal Location:

  3p22.3

• Expression System:

  HEK293T

• Tag:

  C-Myc/DDK

• Related Pathways:

  Endocytosis

• Field of Research:

  Endocytosis

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

• Function:

  Druggable Genome

• Molecular Weight:

  95.8 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  PDCD6IP

• Species:

  Human

• Protein ID:

  NP_037506

• Overview:

  Recombinant protein of human programmed cell death 6 interacting protein (PDCD6IP)

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/10015

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q8WUM4

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_013374

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_037506