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G-361 Lysate

CAT: 0112-ATGL0012-200Size: 200 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGL0012-200Size:200 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
G-361 cells are established from a malignant melanoma of a 31 year-old male. The cells produce melanin for up to 50 population doublings. The Y chromosome could not be detected in this cell line by short tandem repeat (STR) -PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Antigen Species
Human
Applications
WB
Concentration
2 mg/mL (determined by BCA assay)
Additionnal Information
G361, ATGL0012-10 µg, ATGL0012-20 µg, ATGL0012-50 µg, ATGL0012-100 µg, ATGL0012-250 µg, ATGL0012-500 µg, ATGL0012-1 mg, ATGL0012-10, ATGL0012-20, ATGL0012-50, ATGL0012-100, ATGL0012-250, ATGL0012-500, ATGL0012-1
Storage Conditions
Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Scientific Category
Lysates
Species
Human
WB Description
All lanes : G-361 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;
Preparation
Heat lysate to 95°C for 5 minutes and rapidly cooling before use.
Lysate QC
Western Blot analyzed by both GAPDH and Beta tubulin antibody

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