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Human STEAP1 antibody

CAT: 0112-ATGA0551-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0551-050Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Six-transmembrane epithelial antigen of the prostate (STEAP) is expressed predominantly in human prostatetissue and in other common malignancies including prostate, bladder, colon, and ovarian carcinomas, and in Ewing's sarcoma, suggesting that it could function as an almost universal tumor antigen. Immunohistochemical analysisindicated STEAP-1 localization at cell-cell junctions of the secretory epithelium of prostate and prostatecancer cells. The favorable expression profiling of STEAP-1 in normal and cancer tissues suggested itspotential use as a target for immunotherapy.
Product Name Alternative
Six transmembrane epithelial antigen of the prostate 1, six transmembrane epithelial antigen of the prostate, PRSS24
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human STEAP1 (1-70aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
J2D2
Conjugation
Unconjugated
Applications
ELISA, WB
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
STEAP1, ATGA0551-10 µg, ATGA0551-20 µg, ATGA0551-50 µg, ATGA0551-100 µg, ATGA0551-250 µg, ATGA0551-500 µg, ATGA0551-1 mg, ATGA0551-10, ATGA0551-20, ATGA0551-50, ATGA0551-100, ATGA0551-250, ATGA0551-500, ATGA0551-1
References & Citations
David A. Rodeberg, et al. (2005) Clin Cancer Res. June11 (12) : 4545-4552.; ; Pia M. Challita-Eid, et al., (2007) Cancer research June 67 (12) : 5798-5805.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Epigenetics and Nuclear Signaling
NCBI Accession Number
NP_036581
Uniprot Accession Number
Q9UHE8
WB Description
The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STEAP1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: U87MG cell lysate;

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