Human STEAP1 antibody

• Description:

  Six-transmembrane epithelial antigen of the prostate (STEAP) is expressed predominantly in human prostatetissue and in other common malignancies including prostate, bladder, colon, and ovarian carcinomas, and in Ewing's sarcoma, suggesting that it could function as an almost universal tumor antigen. Immunohistochemical analysisindicated STEAP-1 localization at cell-cell junctions of the secretory epithelium of prostate and prostatecancer cells. The favorable expression profiling of STEAP-1 in normal and cancer tissues suggested itspotential use as a target for immunotherapy.

• Product Name Alternative:

  Six transmembrane epithelial antigen of the prostate 1, six transmembrane epithelial antigen of the prostate, PRSS24

• Host:

  Mouse

• Antigen Species:

  Human

• Reactivity:

  Human

• Immunogen:

  Recombinant human STEAP1 (1-70aa) purified from E. coli

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  J2D2

• Conjugation:

  Unconjugated

• Applications:

  ELISA, WB

• Purification Method:

  By protein-A affinity chromatography

• Concentration:

  1 mg/mL (determined by BCA assay)

• Additionnal Information:

  STEAP1, ATGA0551-10 µg, ATGA0551-20 µg, ATGA0551-50 µg, ATGA0551-100 µg, ATGA0551-250 µg, ATGA0551-500 µg, ATGA0551-1 mg, ATGA0551-10, ATGA0551-20, ATGA0551-50, ATGA0551-100, ATGA0551-250, ATGA0551-500, ATGA0551-1

• References & Citations:

  David A. Rodeberg, et al. (2005) Clin Cancer Res. June11 (12) : 4545-4552.; ; Pia M. Challita-Eid, et al., (2007) Cancer research June 67 (12) : 5798-5805.

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

• Applications Notes:

  The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

• Scientific Category:

  Epigenetics and Nuclear Signaling

• NCBI Accession Number:

  NP_036581

• Uniprot Accession Number:

  Q9UHE8

• WB Description:

  The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human STEAP1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: U87MG cell lysate;