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Human KAT2A/GCN5 antibody

CAT: 0112-ATGA0527-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0527-100Size:100 µL
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Description
GCN5L2, as known as GCN5 and KAT2A is the use of immunoprecipitated GCN5L2 for acetylation toprovide the additional components important for acetylation. GCN5L2 can be one of the potential enzymesinvolved in acetylation in vivo. GCN5L2 is expressed predominantly in the embryo and newborn and is essentialfor normal embryonic development. Therefore, GCN5L2 appears to be important for differentiation ofembryonic-derived preadipocytes. On the one hand, loss of GCN5L2 leads to high incidence of apoptosis inthe GCN5L2 mutants that begins before the onset of morphological abnormality.
Product Name Alternative
STAF97, PCAF-b, Lysine acetyltransferase 2A, Histone succinyltransferase KAT2A, Histone glutaryltransferase KAT2A, Histone acetyltransferase KAT2A, Histone acetyltransferase GCN5, General control of amino-acid synthesis 5-like 2, General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human GCN5L2 (411-837aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
AT3G13
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
STAF97, PCAF-b, Lysine acetyltransferase 2A, KAT2A, K (lysine) acetyltransferase 2A, Histone succinyltransferase KAT2A, Histone glutaryltransferase KAT2A, Histone acetyltransferase KAT2A, Histone acetyltransferase GCN5, hGCN5L2, hGCN5, General control of amino-acid synthesis 5-like 2, General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5, ATGA0527-10 µg, ATGA0527-20 µg, ATGA0527-50 µg, ATGA0527-100 µg, ATGA0527-250 µg, ATGA0527-500 µg, ATGA0527-1 mg, ATGA0527-010, ATGA0527-020, ATGA0527-050, ATGA0527-100, ATGA0527-250, ATGA0527-500, ATGA0527-01M
References & Citations
Wiper-Bergeron N, et al. (2007) Proc Natl Acad Sci U S A, 104 (8) :2703-8.; ; Jacob AL, et al. (2001) J Biol Chem, 276 (40) :37659-64.; ; Xu W, et al. (2000) Nat Genet, 26 (2) :229-232.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results
Scientific Category
Epigenetics and Nuclear Signaling
NCBI Accession Number
NP_066564
Uniprot Accession Number
Q92830
WB Description
The cell lysate (35ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GCN5L2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate;
IF Description
ICC/IF analysis of GCN5L2 in HeLa cells. The cell was stained with ATGA0527 (1:500) . The secondary antibody (green) was used Alexa Fluor 488.

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