Human RAB7A antibody

CAT: 0112-ATGA0471-100Size: 100 µLDry Ice: NoHazardous: No
CAT#:0112-ATGA0471-100Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Ras-related protein Rab-7a, also known as RAB7A, is a member of the Ras superfamily of small Rab GTPases. RAB7A associates with the RIPL effector protein to control membrane trafficking from early to late endosome and to lysosomes. RAB7A has also been shown to regulate growth receptor endocytic trafficking, degradation, maturation of phagosome and autophagic vacuoles.
Product Name Alternative
Ras-related protein Rab-7a, RAB7, RAB7A member RAS oncogene family, CMT2B, Charcot-Marie-Tooth neuropathy 2B
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human RAB7A (1-207aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
AT10E4
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
Rab7a, ATGA0471-10 µg, ATGA0471-20 µg, ATGA0471-50 µg, ATGA0471-100 µg, ATGA0471-250 µg, ATGA0471-500 µg, ATGA0471-1 mg, ATGA0471-10, ATGA0471-20, ATGA0471-50, ATGA0471-100, ATGA0471-250, ATGA0471-500, ATGA0471-1
References & Citations
Feng Y., et al. (1995) J Cell Biol. 131: 1435-52.; ; Barbero P., et al. (2002) J Cell Biol. 156: 511-8.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Tags & Cell Markers
NCBI Accession Number
NP_004628
Uniprot Accession Number
P51149
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAB7A antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: Jurkat cell lysate; ; Lane 3.: MCF7 cell lysate; ; Lane 4.: PC3 cell lysate; ; Lane 5.: 293T cell lysate; ; Lane 6.: SK-OV-3 cell lysate
FACS Description
Flow cytometry analysis of RAB7A in HeLa cells. The cell was stained with ATGA0471 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .
IF Description
ICC/IF analysis of RAB7A in HeLa cells. The cell was stained with ATGA0471 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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