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Human SSU72 antibody

CAT: 0112-ATGA0469-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0469-100Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SSU72 RNA polymerase II CTD phosphatase homolog, also known as SSU72, is a highly conserved homologue of yeast Ssu72, a CTD phosphatase and a component of the polyadenylation/ termination machinery. Existing as multiple alternatively spliced isoforms, SSU72 interacts with TFIIB, Rb and DNAM-1 and functions to catalyze the dephosphorylation of target proteins, possibly playing a role in RNA processing and termination via dephosphorylation of Pol II.
Product Name Alternative
SSu72 RNA polymerase II CTD phosphatase homolog, HSPC182, PNAS-120
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human SSU72 (1-194aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT66D10
Conjugation
Unconjugated
Applications
ELISA, WB
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
SSU72, ATGA0469-10 µg, ATGA0469-20 µg, ATGA0469-50 µg, ATGA0469-100 µg, ATGA0469-250 µg, ATGA0469-500 µg, ATGA0469-1 mg, ATGA0469-10, ATGA0469-20, ATGA0469-50, ATGA0469-100, ATGA0469-250, ATGA0469-500, ATGA0469-1
References & Citations
Dichtl B., et al. (2002) Mol Cell. 10: 1139-1150.; ; Krishnamurthy S., et al. (2004) Mol Cell. 14: 387-394.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_054907
Uniprot Accession Number
Q9NP77
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SSU72 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Jurkat cell lysate; ; Lane 2.: K562 cell lysate; ; Lane 3.: 293T cell lysate; ; Lane 4.: HepG2 cell lysate; ; Lane 5.: A549 cell lysate; ; Lane 6.: MCF-7 cell lysate; ; Lane 7.: SK-OV-3 cell lysate; ; Lane 8.: LnCap cell lysate; ; Lane 9.: HeLa cell lysate

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