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Human ACSF2 antibody

CAT: 0112-ATGA0464-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0464-100Size:100 µL
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Description
ACSF2 also known as Acyl-CoA synthetase family member 2. Acyl-CoA synthetases are a family of enzymes that catalyze the thioesterification of fatty acids with coenzymeA to form activated intermediates, which play a fundamental role in lipid metabolism and homeostasis of lipid-related processes. ACSF2 are required for complex lipid synthesis, energy production via beta-oxidation, protein acylation and fatty-acid dependent transcriptional regulation. ACSF2 are also necessary for fatty acid import into cells by the process of vectorial acylation.
Product Name Alternative
Acyl-CoA synthetase family member 2 isoform 2, ACSMW, AVYV493
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human ACSF2 (42-615aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT18B4
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
ACSF2, ATGA0464-10 µg, ATGA0464-20 µg, ATGA0464-50 µg, ATGA0464-100 µg, ATGA0464-250 µg, ATGA0464-500 µg, ATGA0464-1 mg, ATGA0464-10, ATGA0464-20, ATGA0464-50, ATGA0464-100, ATGA0464-250, ATGA0464-500, ATGA0464-1
References & Citations
Black PN., et al. (2007) Biochim Biophys Acta. 1771 (9) : 1246-53.; ; Watkins P.A., et al. (2007) J Lipid Res. 48 (12) : 2736-50.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Metabolism
NCBI Accession Number
NP_079425
Uniprot Accession Number
Q96CM8
WB Description
The HepG2 cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACSF2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
IF Description
ICC/IF analysis of ACSF2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACSF2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)

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