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Human Leptin/OB antibody

CAT: 0112-ATGA0460-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0460-050Size:50 µL
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Description
Human leptin is a 16-kDa nonglycosylated hormone that is produced in mature adipocytes. Leptin acts primarily in the hypothalamus to reduce food intake and body weight. In ob/ob mice, the gene encoding leptin is mutated, resulting in morbid obesity and associated abnormalities, including hyperphagia, hypothermia, diabetes and infertility. The function of leptin in regulating appetite and metabolism, as well as the possibility of using leptin as a therapeutic agent, are currently under intense investigation.
Product Name Alternative
LEP, OB, OBS, Leptin, Obesity factor, Obese protein, Leptin, Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, Obesity, Obesity homolog mouse, Obesity Murine Homolog Leptin
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human Leptin (22-167aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT7E10
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
Leptin, OB, ATGA0460-10 µg, ATGA0460-20 µg, ATGA0460-50 µg, ATGA0460-100 µg, ATGA0460-250 µg, ATGA0460-500 µg, ATGA0460-1 mg, ATGA0460-10, ATGA0460-20, ATGA0460-50, ATGA0460-100, ATGA0460-250, ATGA0460-500, ATGA0460-1
References & Citations
Anini Y., et al. (2003) Diabetes. 52 (2) : 252-259.; ; Jeong KJ., et al. (1999) Appl Environ Microbiol. 65 (7) : 3027-32.; ; Hyogo H., et al. (2002) J. Biol. Chem. 277 (37) : 34117-34124.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_000221
Uniprot Accession Number
P41159
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Leptin antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: Leptin transfected 293T cell lysate
IF Description
ICC/IF analysis of Leptin in HeLa cells. The cell was stained with ATGA0460 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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