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Recombinant mouse Leptin/OB protein

CAT: 0112-LEP0701-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-LEP0701-500Size:500 µg
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Description
Leptin is a 16-kDa nonglycosylated hormone that is produced in mature adipocytes. Leptin acts primarily in the hypothalamus to reduce food intake and body weight. In ob/ob mice, the gene encoding leptin is mutated, resulting in morbid obesity and associated abnormalities, including hyperphagia, hypothermia, diabetes and infertility. Originally discovered in the mouse, the cDNA for leptin has been so far cloned from a variety of species including human, rat, cow, chicken, pig, sheep and dog. Recombinant mouse leptin was overexpressed as insoluble protein aggregate in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer
Product Name Alternative
LEP, OB, OBS, Leptin Mouse, Obesity factor, Obese protein, Leptin
Expression System
E.coli
Antigen Species
Mouse
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
16 kDa (147aa) confirmed by MALDI-TOF
Additionnal Information
Leptin, OB, LEP, OB, OBS, Leptin Mouse, Obesity factor, Obese protein, Leptin, LEP0701-10 µg, LEP0701-20 µg, LEP0701-50 µg, LEP0701-100 µg, LEP0701-250 µg, LEP0701-500 µg, LEP0701-1 mg, LEP0701-10, LEP0701-20, LEP0701-50, LEP0701-100, LEP0701-250, LEP0701-500, LEP0701-1
References & Citations
Jeong KJ., et al. (1999) Appl Environ Microbiol. 65 (7), 3027-32.; ; Anini Y., et al. (2003) Diabetes. 52 (2), 252-259.; ; Hyogo H., et al. (2002) J. Biol. Chem. 277 (37), 34117-34124.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_032519
Uniprot Accession Number
P41160
Species
Mouse
AA Sequence
MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC

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Alternative Products

CatalogName
PROTP41160-20ugMouse Leptin/OB Recombinant Protein
NBP2-34934-1mgRecombinant Human Leptin/OB Protein
MBS637123-01Leptin, Recombinant, Mouse (Ob, Obese Protein, Obesity Factor)
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L1670-10DLeptin, Recombinant, Mouse (LEP, Ob, Obesity Factor) discontinued
L1670-16S1Leptin, Recombinant, Sheep (Ob, Obese Protein, Obesity Factor)
209065Leptin, Rat, Recombinant (Ob, Obese Protein, Obesity Factor)
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