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Human BPGM antibody

CAT: 0112-ATGA0455-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0455-100Size:100 µL
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Description
Bisphosphoglycerate mutase (BPGM) is an enzyme unique to erythrocytes and placental cells. This protein plays a major role in regulating hemoglobin oxygen affinity as a consequence of controlling 2, 3-BPG concentration. It is responsible for the catalytic synthesis of 2, 3-Bisphosphoglycerate (2, 3-BPG) from 1, 3-BPG. BPGM also has a mutase and a phosphatase function, but these are much less active.
Product Name Alternative
Bisphosphoglycerate mutase, BPG-dependent PGAM, Bisphosphoglycerate mutase 2,3-bisphosphoglycerate mutase, erythrocyte, 2,3-bisphosphoglycerate mutase, 2,3-bisphosphoglycerate synthase, Ab2 098, AI323730 AL022789, BPG dependent PGAM, C86192.
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human BPGM (1-259aa) purified from E. coli.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
AT2E11
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
BPGM, Bisphosphoglycerate mutase, BPG-dependent PGAM, Bisphosphoglycerate mutase 2,3-bisphosphoglycerate mutase, erythrocyte, 2,3-bisphosphoglycerate mutase, 2,3-bisphosphoglycerate synthase, Ab2 098, AI323730 AL022789, BPG dependent PGAM, C86192., ATGA0455-10 µg, ATGA0455-20 µg, ATGA0455-50 µg, ATGA0455-100 µg, ATGA0455-250 µg, ATGA0455-500 µg, ATGA0455-1 mg, ATGA0455-10, ATGA0455-20, ATGA0455-50, ATGA0455-100, ATGA0455-250, ATGA0455-500, ATGA0455-1
References & Citations
Pritlove DC., et al. (2006) Placenta. 27 (8) : 924-7. ; ; Wang Y., et al. (2006) J. Biol. Chem. 281 (51) : 39642-8.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_001715
Uniprot Accession Number
P07738
WB Description
The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BPGM antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: BPGM transfected 293T cell lysate
FACS Description
Flow cytometry analysis of BPGM in HeLa cells. The cell was stained with ATGA0455 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .
IF Description
ICC/IF analysis of BPGM in HeLa cells. The cell was stained with ATGA0445 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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