Human CINP antibody

• Description:

  CINP, also known as Cyclin-dependent kinase 2-interacting protein, belongs to the CINP family. This protein interacts with the components of the replication complex and 2 kinases, CDK2 and CDC7, thereby providing a functional and physical link between CDK2 and CDC7 during firing of the origins of replication.

• Product Name Alternative:

  Cyclin-dependent kinase 2-interacting protein, CDK2-interacting protein

• Host:

  Mouse

• Antigen Species:

  Human

• Reactivity:

  Human

• Immunogen:

  Recombinant human CINP (1-212aa) purified from E. coli

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  AT1G10

• Conjugation:

  Unconjugated

• Applications:

  ELISA, WB

• Purification Method:

  By protein-A affinity chromatography

• Concentration:

  1 mg/mL (determined by BCA assay)

• Additionnal Information:

  CINP, Cyclin-dependent kinase 2-interacting protein, CDK2-interacting protein, ATGA0448-10 µg, ATGA0448-20 µg, ATGA0448-50 µg, ATGA0448-100 µg, ATGA0448-250 µg, ATGA0448-500 µg, ATGA0448-1 mg, ATGA0448-10, ATGA0448-20, ATGA0448-50, ATGA0448-100, ATGA0448-250, ATGA0448-500, ATGA0448-1

• References & Citations:

  Grishina I., et al. (2005) Cell Cycle. 4: 1120-1126.; ; Lovejoy C.A., et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106: 19304-19309.

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation:

  Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

• Applications Notes:

  The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

• Scientific Category:

  Cell Cycle

• NCBI Accession Number:

  NP_116019

• Uniprot Accession Number:

  Q9BW66

• WB Description:

  The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CINP antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Jurkat cell lysate ; ; Lane 2.: K562 cell lysate ; ; Lane 3.: 293T cell lysate ; ; Lane 4.: HepG2 cell lysate ; ; Lane 5.: A549 cell lysate ; ; Lane 6.: MCF7 cell lysate ; ; Lane 7.: LnCap cell lysate ; ; Lane 8.: HeLa cell lysate ; ; Lane 9.: SK-OV-3 cell lysate