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Human Jak2 antibody

CAT: 0112-ATGA0421-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0421-100Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
JAK2 is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis.
Product Name Alternative
Janus kinase 2, Janus kinase 2, Janus kinase 2 (a protein tyrosine kinase), JTK10
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human JAK2 (1014-1132aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT53B7
Conjugation
Unconjugated
Applications
ELISA, WB, FACS, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
Jak2, Janus kinase 2, Janus kinase 2, Janus kinase 2 (a protein tyrosine kinase), JTK10, ATGA0421-10 µg, ATGA0421-20 µg, ATGA0421-50 µg, ATGA0421-100 µg, ATGA0421-250 µg, ATGA0421-500 µg, ATGA0421-1 mg, ATGA0421-10, ATGA0421-20, ATGA0421-50, ATGA0421-100, ATGA0421-250, ATGA0421-500, ATGA0421-1
References & Citations
Bole-Feycot C., et al. (1998) Endocr Rev. 19 (3) : 225-68. doi:10.1210/er.19.3.225.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Cell Cycle
NCBI Accession Number
NP_004963
Uniprot Accession Number
O60674
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human JAK2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : K-562 cell lysate ; ; Lane 2. : TF-1 cell lysate ; ; Lane 3. : 293T cell lysate ; ; Lane 4 : Jurkat cell lysate ; ; Lane 5 : THP-1 cell lysate
FACS Description
Flow cytometry analysis of JAK2 in HeLa cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .
IF Description
ICC/IF analysis of JAK2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human JAK2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .

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