Human ASS1 antibody

CAT: 0112-ATGA0416-100Size: 100 µLDry Ice: NoHazardous: No

1 / 1

CAT#:0112-ATGA0416-100Size:100 µL

Selected

24/48H Stock Items & 2 to 6 Weeks non Stock Items.

Quick Request Actions

Description

ASS1 (argininosuccinate synthase or citrulline-aspartate ligase) belongs to the argininosuccinate synthase family. ASS1 is a urea cycle enzyme that is essential in the conversion of nitrogen from ammonia and aspartate to urea. A deficiency of ASS1 causes citrullinemia (CTLN1), an autosomal recessive disease which is characterized by severe vomiting spells and mental retardation. Cells deficient in ASS1 must absorb the arginine they need for growth from circulating blood.

Product Name Alternative

Argininosuccinate synthase, ASS, CTLN1, Argininosuccinate synthase

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ASS1 (1-412aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT1G11

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ASS1, Argininosuccinate synthase, ASS, CTLN1, Argininosuccinate synthase, ATGA0416-10 µg, ATGA0416-20 µg, ATGA0416-50 µg, ATGA0416-100 µg, ATGA0416-250 µg, ATGA0416-500 µg, ATGA0416-1 mg, ATGA0416-10, ATGA0416-20, ATGA0416-50, ATGA0416-100, ATGA0416-250, ATGA0416-500, ATGA0416-1

References & Citations

Chalikiopoulou C., et al. (2016) Pharmacogenomics. (4) : 393-403.; ; Rabinovich S., et al. (2015) Nature. 527 (7578) : 379-83.; ; Walts AE., et al. (2015) J Cancer Res Clin Oncol. 141 (8) : 1363-9.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000041

Uniprot Accession Number

P00966

WB Description

The MCF-7 cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ASS1 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IF Description

ICC/IF analysis of ASS1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ASS1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0416-IF1b.jpg}

Related Products

Catalog	Name
ATGP0636-010	Recombinant human ASS1 protein
ATGP0636-250	Recombinant human ASS1 protein
ATGP0636-050	Recombinant human ASS1 protein
ATGA0604-100	Human AGXT antibody
ATGA0583-050	Human ACAT1 antibody
ATGA0581-050	Human LSM2 antibody
ATGA0580-050	Human AGXT antibody
ATGA0578-100	Human PARP2 antibody
ATGA0578-050	Human PARP2 antibody
ATGA0577-050	Human PSMD11 antibody

Alternative Products

Catalog	Name
ARO-A11750-01	Anti-Human ASS1 Polyclonal Antibody
P0269	Human, Rat, Mouse ASS1 antibody
MBS2101543-01	Human Argininosuccinate Synthetase 1 (ASS1) Antibody Pair Kit (with Standard)
OM299675-01	ASS1 Human
GTR18158770-01	ASS1 Antibody

Human ASS1 antibody

Related Products

Alternative Products

Popular Products