Products for Research Use Only
RegisterLogin

Human VTA1 antibody

CAT: 0112-ATGA0403-050Size: 50 µLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0112-ATGA0403-050Size:50 µL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
The VTA1 protein is involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Thought to be a cofactor of VPS4A/B, which catalyzes disassembles membrane-associated ESCRT-III assemblies. This protein is involved in the sorting and down-regulation of EGFR (By similarity) and involved in HIV-1 budding.
Product Name Alternative
Vacuolar protein sorting-associated protein VTA1 homolog, DRG-1, DRG1, LIP5, SBP1
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human VTA1 (1-307aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT14G10
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
VTA1, ATGA0403-10 µg, ATGA0403-20 µg, ATGA0403-50 µg, ATGA0403-100 µg, ATGA0403-250 µg, ATGA0403-500 µg, ATGA0403-1 mg, ATGA0403-10, ATGA0403-20, ATGA0403-50, ATGA0403-100, ATGA0403-250, ATGA0403-500, ATGA0403-1
References & Citations
Jun Shi., et al. (2001) Brain Res. 910 (1-2) : 29-37. ; ; Fujita H., et al. (2004) J Cell Sci. 117 (Pt 14) : 2997-3009.; ; Ward DM., et al. (2005) J Biol Chem. 280 (11) : 10548-55.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Virus
NCBI Accession Number
NP_057569
Uniprot Accession Number
Q9NP79
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VTA1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: 293T cell lysate; ; Lane 3.: HeLa cell lysate; ; Lane 4.: MCF7 cell lysate; ; Lane 5.: A549 cell lysate; ; Lane 6.: Jurkat cell lysate; ; Lane 7.: K562 cell lysate; ; Lane 8.: LnCaP cell lysate; ; Lane 9.: U937 cell lysate; ; Lane 10.: A431 cell lysate
FACS Description
Flow cytometry analysis of VTA1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .
IF Description
ICC/IF analysis of VTA1 in Hep3B cells. The cell was stained with ATGA0403 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

Related Products

CatalogName
ATGP1419-020Recombinant human VTA1 protein
ATGP1419-500Recombinant human VTA1 protein
ATGP1419-100Recombinant human VTA1 protein
ATGA0604-100Human AGXT antibody
ATGA0583-050Human ACAT1 antibody
ATGA0581-050Human LSM2 antibody
ATGA0580-050Human AGXT antibody
ATGA0578-100Human PARP2 antibody
ATGA0578-050Human PARP2 antibody
ATGA0577-050Human PSMD11 antibody

Alternative Products

CatalogName
32151-05111Human Vacuolar Protein Sorting-Associated Protein (VTA1) Homolog Antibody
32151-05121Human Vacuolar Protein Sorting-Associated Protein (VTA1) Homolog Antibody (Biotin Conjugate)
32151-05151Human Vacuolar Protein Sorting-Associated Protein (VTA1) Homolog AssayLite™ Antibody (RPE Conjugate)
32151-05141Human Vacuolar Protein Sorting-Associated Protein (VTA1) Homolog AssayLite™ Antibody (FITC Conjugate)
32151-05161Human Vacuolar Protein Sorting-Associated Protein (VTA1) Homolog AssayLite™ Antibody (APC Conjugate)
32151-05171Human Vacuolar Protein Sorting-Associated Protein (VTA1) Homolog AssayLite™ Antibody (PerCP Conjugate)
PT-A05925-01VTA1 Human
70R-21284VTA1 antibody

Popular Products