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Human AHSA1 antibody

CAT: 0112-ATGA0388-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0388-100Size:100 µL
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Description
The Co-chaperone AHA1 (Activator of HSP90 ATPase-1) is proposed to function as an ATPase activating protein. May affect a step in the endoplasmic reticulum to Golgi trafficking. It expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta.
Product Name Alternative
Activator of Hsp90 ATPase-1 (AHA1, 19-337), AHA1, AHSA1, Activator of Hsp90 ATPase protein 1, Hsp90 co-chaperone AHA1, Activator of Hsp90 ATPase-1 Activator of 90 kDa heat shock protein ATPase homolog 1, Activator of heat shock 90kDa protein ATPase homolog 1, C14orf3, HSPC322, p38
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human AHA1 (19-337aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT3E9
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
AHA1, Activator of Hsp90 ATPase-1, AHA1, Activator of Hsp90 ATPase-1 (AHA1, 19-337), Activator of Hsp90 ATPase protein 1, Hsp90 co-chaperone AHA1, Activator of Hsp90 ATPase-1 Activator of 90 kDa heat shock protein ATPase homolog 1, Activator of heat shock 90kDa protein ATPase homolog 1, AHA 1, AHSA 1, AHSA1, C14orf3, HSPC322, p38, ATGA0388-10 µg, ATGA0388-20 µg, ATGA0388-50 µg, ATGA0388-100 µg, ATGA0388-250 µg, ATGA0388-500 µg, ATGA0388-1 mg, ATGA0388-010, ATGA0388-020, ATGA0388-050, ATGA0388-100, ATGA0388-250, ATGA0388-500, ATGA0388-01M
References & Citations
Meyer P., et al. (2004) EMBO. 23 (6) : 1402-1410. ; ; Siligardi G., et al. (2004) J Biol Chem. 270 (50) : 51989-51998.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution for Western blot analysis is 1:1000.
Scientific Category
Heat Shock Proteins
NCBI Accession Number
NP_036243
Uniprot Accession Number
O95433
WB Description
The Recombinant Human AHA1 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AHA1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : AHA1 Recombinant protein ; ; Lane 2. : MCF-7 cell lysate ; ; Lane 3. : PC-3 cell lysate ; ; Lane 4. : 293T cell lysate ; ; Lane 5. : HeLa cell lysate
IF Description
ICC/IF analysis of AHA1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AHA1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . ICC/IF analysis of AHA1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AHA1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0388-IF2.jpg}

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