Human BCAT1 antibody

CAT: 0112-ATGA0359-100Size: 100 µLDry Ice: NoHazardous: No
CAT#:0112-ATGA0359-100Size:100 µL
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Description
BCAT1, also known as branched chain amino-acid transaminase (cytosolic form), catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. BCAT1 is expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma.
Product Name Alternative
Branched chain amino-acid transaminase cytosolic, Branched chain amino-acid transaminase, cytosolic, BCATC, BCT1, ECA39, MECA39, PNAS121, PP18
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human BCAT1 (1-386aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT3C8
Conjugation
Unconjugated
Applications
ELISA, WB
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
BCAT1, ATGA0359-10 µg, ATGA0359-20 µg, ATGA0359-50 µg, ATGA0359-100 µg, ATGA0359-250 µg, ATGA0359-500 µg, ATGA0359-1 mg, ATGA0359-10, ATGA0359-20, ATGA0359-50, ATGA0359-100, ATGA0359-250, ATGA0359-500, ATGA0359-1
References & Citations
Goto M., et al. (2005) J Biol Chem. 280: 37246-37256.; ; Schuldiner O., et al. (1996) Proc Natl Acad Sci USA. 93: 7143-7148.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_005495
Uniprot Accession Number
P54687
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BCAT1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : Jurkat cell lysate; ; Lane 2. : HeLa cell lysate

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