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Recombinant human BCAT2 protein

CAT: 0112-ATGP1523-010Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP1523-010Size:10 µg
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Description
BCAT2 belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. This protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. It may also function as a transporter of branched chain alpha-keto acids. Recombinant human BCAT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Branched chain amino-acid aminotransferase 2 mitochondrial, Branched chain amino-acid aminotransferase 2, mitochondrial, BCAM, BCATM, BCT2, PP18, ECA40, Placental protein 18
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
43.9 kDa (390aa) confirmed by MALDI-TOF
Additionnal Information
BCAT2, Branched chain amino-acid aminotransferase 2 mitochondrial, Branched chain amino-acid aminotransferase 2, mitochondrial, BCAM, BCATM, BCT2, PP18, ATGP1523-10 µg, ATGP1523-20 µg, ATGP1523-50 µg, ATGP1523-100 µg, ATGP1523-250 µg, ATGP1523-500 µg, ATGP1523-1 mg, ATGP1523-10, ATGP1523-20, ATGP1523-50, ATGP1523-100, ATGP1523-250, ATGP1523-500, ATGP1523-1
References & Citations
Coles SJ. et al. (2009) Biochemistry Jan 27; ;48 (3) :645-56.; ; Yennawar N.H.et al. (2007) J. Biol. Chem. 281:39660-39671
Other References
Günther J, et al. BAY-069, a Novel (Trifluoromethyl) pyrimidinedione-Based BCAT1/2 Inhibitor and Chemical Probe. (J Med Chem. 2022) {https://pubmed.ncbi.nlm.nih.gov/36261130/}
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol 0.2M NaCl, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_001181
Uniprot Accession Number
O15382
Species
Human
AA Sequence
ASSSF KAADLQLEMT QKPHKKPGPG EPLVFGKTFT DHMLMVEWND KGWGQPRIQP FQNLTLHPAS SSLHYSLQLF EGMKAFKGKD QQVRLFRPWL NMDRMLRSAM RLCLPSFDKL ELLECIRRLI EVDKDWVPDA AGTSLYVRPV LIGNEPSLGV SQPTRALLFV ILCPVGAYFP GGSVTPVSLL ADPAFIRAWV GGVGNYKLGG NYGPTVLVQQ EALKRGCEQV LWLYGPDHQL TEVGTMNIFV YWTHEDGVLE LVTPPLNGVI LPGVVRQSLL DMAQTWGEFR VVERTITMKQ LLRALEEGRV REVFGSGTAC QVCPVHRILY KDRNLHIPTM ENGPELILRF QKELKEIQYG IRAHEWMFPV

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